Anabolic response of mouse bone-marrow-derived stromal cell clone ST2 cells to low-intensity pulsed ultrasound
Anabolic response of mouse bone-marrow-derived stromal cell clone ST2 cells to low-intensity pulsed ultrasound
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DOI：
10.1006/bbrc.2000.2094
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发表时间：
2000-02-05
影响因子：
3.1
通讯作者：
Itoman, M
中科院分区：
文献类型：
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作者：
Naruse, K;Mikuni-Takagaki, Y;Itoman, M
在骨髓基质来源的ST 2细胞中研究了暴露于低强度脉冲超声20分钟的影响。他们对超声波的反应是IGF mRNA、骨钙素和骨唾液蛋白mRNA水平升高。这些信息的上调表达以双相方式出现，第一个峰对蛋白质合成抑制剂放线菌酮具有抗性，第二个峰被诱导性前列腺素G/H合酶（环氧合酶-2）的抑制剂NS398消除。在体内观察到的机械负荷的累积效应称为记忆效应，可以从前列腺素介导的这种双相合成代谢反应中解释。IGF或骨钙素mRNA的上调，甚至可以观察到在24小时后开始的20分钟暴露于超声波。我们的研究结果表明，这种低强度的脉冲超声波，已在临床上用于加速骨折愈合过程，诱导成骨细胞的直接合成代谢反应，导致骨基质形成。(C)北京大学出版社.
The effects of 20-min exposure to low-intensity, pulsed ultrasound were investigated in ST2 cells of bone marrow stromal origin. They responded to ultrasound with elevated levels of IGF mRNAs, osteocalcin, and bone sialoprotein mRNAs. The upregulated expression of these messages appeared in a biphasic manner, with the first peak resistant to the protein synthesis inhibitor cycloheximide, and a second peak that was eliminated by NS398, an inhibitor of the inducive prostaglandin G/H synthase (cyclooxygenase-2). A cumulative effect of mechanical loading called the memory effect, which has been observed in vivo, can be explained from such a biphasic anabolic reaction mediated by prostaglandins. The upregulation of IGF or osteocalcin mRNAs can be observed even at 24 h after the initiation of the 20-min exposure to ultrasound. Our results suggest that this low-intensity, pulsed ultrasound, which has been clinically used to accelerate the healing processes of fractured bone, induces a direct anabolic reaction of osteogenic cells, leading to bone matrix formation. (C) 2000 Academic Press.