Ultrasensitive and simultaneous determination of RNA modified nucleotides by sheathless interfaced capillary electrophoresis-tandem mass spectrometry
Ultrasensitive and simultaneous determination of RNA modified nucleotides by sheathless interfaced capillary electrophoresis-tandem mass spectrometry
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无鞘接口毛细管电泳-串联质谱法超灵敏同时测定 RNA 修饰核苷酸
DOI：
10.1039/c9cc03195b
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发表时间：
2019
影响因子：
4.9
通讯作者：
Zhang Xin-Xiang
中科院分区：
文献类型：
--
作者：
Yu Yue;Zhu Si-Hao;Yuan Fang;Zhang Xiao-Hui;Lu Yan-Ye;Zhou Ying-Lin;Zhang Xin-Xiang
首次建立了基于无鞘毛细管电泳-串联质谱系统的无标记超灵敏同时测定八个RNA修饰核苷酸。该系统仅使用 500 pg–5 ng 实用 RNA 样品就表现良好，并且随着镍浓度的增加，观察到 HCT 116 细胞中大多数目标核苷酸呈下降趋势。
A label-free ultrasensitive determination of eight RNA modified nucleotides simultaneously was first established based on a sheathless capillary electrophoresis–tandem mass spectrometry system. This system performed well using only 500 pg–5 ng practical RNA samples, and a downward trend of most target nucleotides in HCT 116 cells was observed with the increase of nickel concentration.