Multimodal Functional Analysis Platform: 1. Ultrathin Fluorescence Endoscope Imaging System Enables Flexible Functional Brain Imaging
Multimodal Functional Analysis Platform: 1. Ultrathin Fluorescence Endoscope Imaging System Enables Flexible Functional Brain Imaging
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DOI：
10.1007/978-981-15-8763-4_31
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发表时间：
2021-01-01
期刊：
影响因子：
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通讯作者：
Mushiake，Hajime
中科院分区：
文献类型：
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作者：
Osanai，Makoto;Miwa，Hideki;Mushiake，Hajime
为了阐明脑功能的表达机制，我们开发了一种荧光内窥镜成像系统（U-FEIS），该系统可以在任何深度对脑中的细胞进行成像，同时最大限度地减少侵入。U-FEIS的内窥镜部分由GRIN透镜和直径为450 μm的10，000像素图像光纤组成。U-FEIS的专用显微镜面积在30 cm见方，包括为内窥镜优化的透镜和滤光片。使用U-FEIS，我们成功地可视化表达GFP的神经元与单细胞分辨率和记录的多神经元活动在体外和体内。U-FEIS还可以同时进行成像和光学刺激。因此，U-FEIS应该是神经科学研究中一个强有力的光学工具。
To elucidate the expression mechanisms of brain functions, we have developed an ultrathin fluorescence endoscope imaging system (U-FEIS) that can image cells in the brain at any depth while minimizing the invasion. The endoscope part of U-FEIS consists of a GRIN lens and a 10,000-pixel image fiber with a diameter of 450 μm. The specialized microscope of U-FEIS is within 30 cm square and includes lenses and optical filters optimized for the endoscope. Using U-FEIS, we successfully visualized neurons expressing GFP with single-cell resolution and recorded the multineuronal activities in vitro and in vivo. U-FEIS can also perform imaging and optical stimulation simultaneously. Therefore, U-FEIS should be a powerful optical tool in neuroscience research.