THE ROLE OF THE 9TH AND 10TH TYPE-III DOMAINS OF HUMAN FIBRONECTIN IN CELL-ADHESION
THE ROLE OF THE 9TH AND 10TH TYPE-III DOMAINS OF HUMAN FIBRONECTIN IN CELL-ADHESION
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DOI：
10.1016/0014-5793(94)80137-1
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发表时间：
1994-03-07
期刊：
影响因子：
3.5
通讯作者：
GRANT, KE
中科院分区：
文献类型：
--
作者：
MARDON, HJ;GRANT, KE
纤维连接蛋白(FN)除了在第十个III型结构域(FIII10)上含有细胞识别位点RGD外，还含有黏附活性所必需的位点。分析了FIII10和邻近的FIII9在功能细胞黏附实验中的作用，重组FIII域的结构域边界是严格保守的。FIII9没有粘附性。FIII10和FIII9+FIII10的活性低于FN，而FIII9-10的活性与FN相似。我们得出结论，FIII9与FIII10在细胞黏附方面具有协同作用，这种协同作用依赖于FIII9-10结构域对的结构完整性。
Fibronectins (FN) contain sites, in addition to the cell recognition site RGD in the tenth type III domain (FIII10), that are required for adhesive activity. The role of FIII10 and the adjacent FIII9 was analysed in functional cell adhesion assays recombinant FIII domains in which the domain boundaries were strictly conserved. FIII9 had no adhesive activity. FIII10, and FIII9 plus FIII10 had less activity than FN, whereas the activity of FIII9-10 was similar to FN. We conclude that FIII9 acts synergistically with FIII10 in cell adhesion, and that this synergy is dependent upon the structural integrity of the FIII9-10 pair of domains.