An inhalation anesthetic device for stereotaxic operation on mouse pups
An inhalation anesthetic device for stereotaxic operation on mouse pups
复制标题
一种用于幼鼠立体定位手术的吸入麻醉装置
DOI：
10.1016/j.jneumeth.2015.01.025
复制
发表时间：
2015
影响因子：
3
通讯作者：
Shoji Takeuchi
中科院分区：
文献类型：
--
作者：
Sachine Yoshida1;2;Yuya Morimoto;Taishi Tonooka;Shoji Takeuchi
小鼠幼崽是理解行为发展背后的分子和神经基础的宝贵模型动物。立体定向手术与麻醉控制是系统神经科学的有用工具。然而，没有商业上可用的麻醉剂或立体定向装置用于小鼠幼崽。目前的设备存在一些问题，如侵入式入路稳定，卫生控制差，以及与其他手术设备结合的灵活性较差。本研究以聚二甲基硅氧烷（PDMS）为原料，研制了一种具有立体定向功能的小鼠吸入麻醉装置。PDMS耐高温和耐水，并且足够柔软，可以切割或打洞。麻醉药和立体定向部件是根据真实小鼠幼鼠头部的三维计算机辅助设计（3D CAD）数据制作的。结果将示踪剂注入大脑以证实其效用。我们能够使用PDMS装置以非侵入性的方式立即麻醉和稳定幼崽。组织学染色显示示踪剂注射成功。我们的设备分别通过修剪和管插入与各种类型的商用立体定向和麻醉设备兼容。与现有方法相比，据我们所知，这是首次报道一种以无创方式稳定小鼠幼仔头部的装置，并可作为一种可消毒的吸入麻醉装置。结论该方法为不同发育阶段的动物模型立体定向手术提供了方便实用的工具。
BackgroundMouse pups are invaluable model animals for understanding the molecular and neural basis underlying behavioral development. Stereotaxic operations with anesthetic control are useful tools in systems neuroscience. However, there are no commercially available anesthetic or stereotaxic devices for mouse pups. Current devices have several problems such as invasive approach for stabilization, poor sanitary control, and less flexibility to combine other surgical apparatuses.New MethodHere, we developed an inhalation anesthetic device equipped with stereotaxic function for mouse pups, by using polydimethylsiloxane (PDMS). PDMS is tolerant to heat and water exposure, and soft enough to cut or make a hole. The anesthetic and the stereotaxic parts were fabricated from the three-dimensional computer-aided design (3D CAD) data obtained from the head of a real mouse pup.ResultsTo confirm its utility, a tracer was injected into the brain. We were able to anesthetize and stabilize pups at once in a non-invasive manner using the PDMS device. The histological staining revealed that tracer injection was successful. Our device was compatible with various types of commercial stereotaxic and anesthetic apparatuses via trimming and tube insertion, respectively.Comparison with existing method(s)To our knowledge, this is the first report of a device that can stabilize the mouse pup's head with the non-invasive manner and functions as an inhalation anesthetic device that can be sterilized.ConclusionsThe present fabrication method will provide a handy and functional instrument for stereotaxic operations in animal models at various developmental stages.