Proteomics Reveals the Molecular Underpinnings of Stronger Learning and Memory in Eastern Compared to Western Bees.
Proteomics Reveals the Molecular Underpinnings of Stronger Learning and Memory in Eastern Compared to Western Bees.
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DOI：
10.1074/mcp.ra117.000159
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
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作者：
Meng L;Huo X;Feng M;Fang Y;Han B;Hu H;Wu F;Li J
东方蜜蜂(Apis cerana cerana，ACC)和西方蜜蜂(Apis Melvena Ligustica，AML)是蜜蜂的两个主要种类。令人惊讶的是，对ACC和AML脑亚器官的基本分子神经生物学知之甚少。我们对这两个物种大脑中的蘑菇体(MBS)、触角叶(ALS)和光学叶(OL)的蛋白质组进行了描述和比较，并从生物学上验证了与学习和记忆相关的功能。ACC和AML在MBS和OL中进化出相似的蛋白质组特征，以驱动特定区域的神经活动。在两种动物的MBS中，与ALS和OLS相比，与蛋白质代谢和钙转运相关的功能基团普遍丰富和增强，这表明蛋白质和钙在通过调节突触结构和信号转导来巩固学习和记忆方面是至关重要的。此外，在两个物种的OL中，主要富含核糖核苷的代谢表明它作为第二信使在促进光转导方面发挥了至关重要的作用。值得注意的是，在这两个物种的肌萎缩侧索硬化症中，不同的蛋白质组设置形成了最佳的嗅觉学习和记忆。在ACC的ALS中，这是由丰富的细胞骨架组织支持的，通过调节肾小球的可塑性和细胞内运输来维持嗅觉信号。然而，在AML的ALS中，与氢离子运输有关的丰富的官能团表明，它们通过调节突触传递来支持嗅觉过程。与AML相比，ACC的ALS和MBS的蛋白质代谢和信号转导活性增强的生物学证实表明ACC具有更强的嗅觉学习和记忆能力。已报道的第一个关于蜜蜂脑亚器官的深入蛋白质组数据为神经生物学的分子基础提供了新的见解，并可能对蜜蜂和其他昆虫的进一步神经学研究有用。
The eastern (Apis cerana cerana, Acc) and western (Apis mellifera ligustica, Aml) honeybee are two major honeybee species. Surprisingly, little is known about the fundamental molecular neurobiology of brain suborgans of Acc and Aml. We characterized and compared the proteomes of mushroom bodies (MBs), antennal lobes (ALs) and optical lobes (OLs) in the brain of both species, and biologically validated the functions related to learning and memory. Acc and Aml have evolved similar proteome signatures in MBs and OLs to drive the domain-specific neural activities. In MBs of both species, commonly enriched and enhanced functional groups related to protein metabolism and Ca2+ transport relative to ALs and OLs, suggests that proteins and Ca2+ are vital for consolidating learning and memory via modulation of synaptic structure and signal transduction. Furthermore, in OLs of both species, the mainly enriched ribonucleoside metabolism suggests its vital role as second messenger in promoting phototransduction. Notably, in ALs of both species, distinct proteome settings have shaped to prime olfactory learning and memory. In ALs of Acc, this is supported by the enriched cytoskeleton organization to sustain olfactory signaling through modulation of plasticity in glomeruli and intracellular transport. In ALs of Aml, however, the enriched functional groups implicated in hydrogen ion transport are indicative of their importance in supporting olfactory processes by regulation of synaptic transmission. The biological confirmation of enhanced activities of protein metabolism and signal transduction in ALs and MBs of Acc relative to in Aml demonstrates that a stronger sense of olfactory learning and memory has evolved in Acc. The reported first in-depth proteome data of honeybee brain suborgans provide a novel insight into the molecular basis of neurobiology, and is potentially useful for further neurological studies in honeybees and other insects.