AgDscam, a hypervariable immunoglobulin domain-containing receptor of the Anopheles gambiae innate immune system.
AgDscam, a hypervariable immunoglobulin domain-containing receptor of the Anopheles gambiae innate immune system.
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DOI：
10.1371/journal.pbio.0040229
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发表时间：
2006-07
期刊：
影响因子：
9.8
通讯作者：
Dimopoulos, George
中科院分区：
文献类型：
--
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
昆虫先天免疫系统的激活依赖于能够与病原体相关分子模式相互作用的有限数量的模式识别受体（PRR）。在这里，我们报告了一种新的作用，选择性剪接高变免疫球蛋白结构域编码基因，Dscam，在产生广泛的PRR涉及免疫防御的疟疾载体冈比亚按蚊。蚊子唐氏综合征细胞粘附分子基因AgDscam具有复杂的基因组结构，具有101个外显子，可以产生超过31，000种潜在的选择性剪接形式，具有不同的粘附结构域和相互作用特异性组合。AgDscam通过产生病原体挑战特异性剪接形式谱系来响应感染。AgDscam的瞬时沉默损害了蚊子对细菌和疟原虫感染的抵抗力。AgDscam介导细菌的吞噬作用，它可以以剪接形式特异性的方式与细菌结合并防御细菌。AgDscam是A.冈比亚先天免疫系统。 AgDscam有101个外显子，可以产生超过31，000种潜在的选择性剪接形式。特定的剪接形式产生的感染响应于给定的病原体有助于蚊子的抵抗病原体。
Activation of the insect innate immune system is dependent on a limited number of pattern recognition receptors (PRRs) capable of interacting with pathogen-associated molecular pattern. Here we report a novel role of an alternatively spliced hypervariable immunoglobulin domain-encoding gene, Dscam, in generating a broad range of PRRs implicated in immune defense in the malaria vector Anopheles gambiae. The mosquito Down syndrome cell adhesion molecule gene, AgDscam, has a complex genome organization with 101 exons that can produce over 31,000 potential alternative splice forms with different combinations of adhesive domains and interaction specificities. AgDscam responds to infection by producing pathogen challenge-specific splice form repertoires. Transient silencing of AgDscam compromises the mosquito's resistance to infections with bacteria and the malaria parasite Plasmodium. AgDscam is mediating phagocytosis of bacteria with which it can associate and defend against in a splice form–specific manner. AgDscam is a hypervariable PRR of the A. gambiae innate immune system. AgDscam has 101 exons that can produce more than 31,000 potential alternative splice forms. Specific splice forms produced in response to infection by a given pathogen contribute to the mosquito's resistance against pathogens.