Nucleotide sequence variations may be associated with virulence of deformed wing virus
Nucleotide sequence variations may be associated with virulence of deformed wing virus
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DOI：
10.1007/s13592-019-00660-5
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发表时间：
2019-08-01
期刊：
影响因子：
2.4
通讯作者：
Manuel Sanchez-Vizcaino, Jose
中科院分区：
文献类型：
--
作者：
Barroso-Arevalo, Sandra;Vicente-Rubiano, Marina;Manuel Sanchez-Vizcaino, Jose
西方蜜蜂（Apis mellifera）是作物授粉和维护全球生物多样性的关键角色。它们的生存能力受到狄斯瓦螨的威胁，狄斯瓦螨是变形翅病毒（DWV）的载体。已经报道了几种遗传DWV变体，但尚不清楚它们的毒力是否不同。我们在21个月内检查了两个殖民地中的V. destructor和DWV的患病率以及蜜蜂健康状况，然后使用遗传学方法表征每个殖民地的DWV变体。菌落H没有显示出疾病或死亡的迹象，并且来自该菌落的DWV序列与先前在健康菌落中报道的VDV/DWV-B序列聚类。菌落W显示DWV症状，并且DWV序列与先前在具有症状的菌落中报道的DWV-A序列聚类。这些结果表明，DWV基因组中的核苷酸变异可以影响其毒力。对菌落中的DWV变异体进行基因分型可能是评估风险和早期采取预防措施的有效工具。
Western honey bees (Apis mellifera) are key players in crop pollination and in the maintenance of global biodiversity. Their viability is threatened by Varroa destructor, which acts as a vector of the deformed wing virus (DWV). Several genetic DWV variants have been reported, but it is unclear whether their virulence differs. We examined the prevalence of V. destructor and DWV as well as bee health in two colonies over 21 months and then characterizing DWV variants from each colony using phylogenetics. Colony H showed no signs of disease or mortality, and DWV sequence from this colony clustered with VDV/DWV-B sequences previously reported in healthy colonies. Colony W showed DWV symptoms, and DWV sequence clustered with DWV-A sequences previously reported in colonies with symptoms. These results suggest that nucleotide variations in the DWV genome can affect its virulence. Genotyping DWV variants in colonies may be an effective tool to assess risk and initiate preventive measures early.