Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold
Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold
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DOI：
10.1016/j.biomaterials.2005.12.005
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发表时间：
2006-05-01
期刊：
影响因子：
14
通讯作者：
Shinomiya, K
中科院分区：
文献类型：
--
作者：
Wang, JY;Asou, Y;Shinomiya, K
为了在多孔陶瓷与骨髓基质细胞（BMSCs）的复合物中获得更广泛的骨形成，我们假设低压系统有助于促进更多的BMSCs灌注到多孔支架中，从而增强复合物内的骨形成。在成骨培养基中培养BMSCs后，将β - 磷酸三钙（β - TCP）多孔块浸泡在细胞悬液中。将多孔块与BMSCs的复合物立即放入真空干燥器中。对低压组施加低压，而对照组则保持在大气压下。将复合物在体外培养或皮下植入同系大鼠体内，然后进行生物学和组织学分析。在体外组中，低压组的β - TCP块中的细胞悬液体积、细胞接种效率、碱性磷酸酶（ALP）活性和DNA含量均显著高于对照组。扫描电子显微镜（SEM）显示，低压组中更多的细胞覆盖在复合物的中心部分。在植入大鼠体内3周和6周后，复合物中的ALP活性升高。组织形态计量学分析表明，低压组比对照组有更均匀和广泛的骨形成。在将BMSCs在灌注培养基中接种到多孔支架期间施加低压对组织工程骨形成是有用的。（c）2005爱思唯尔有限公司。保留所有权利。
To obtain more extensive bone formation in composites of porous ceramics and bone marrow stromal cells (BMSCs), we hypothesized that a low-pressure system would serve to facilitate the perfusion of larger number of BMSCs into the porous scaffold, enhancing bone formation within the composites. After culturing BMSCs in osteogenic medium, porous blocks of beta-tricalcium phosphate (beta-TCP) were soaked in the cell suspension. Composites of the block and BMSCs were put immediately into a vacuum desiccator. Low pressure was applied to the low pressure group, while controls were left at atmospheric pressure. Composites were incubated in vitro or subcutaneously implanted into syngeneic rats, then analyzed biologically and histologically. In the in vitro group, cell suspension volume, cell seeding efficiency, alkaline phosphatase (ALP) activity, and DNA content in the beta-TCP blocks were significantly higher in low pressure group than in the controls. Scanning electron microscopy (SEM) demonstrated that a greater number of cells covered the central parts of the composites in the low pressure group. ALP activity in the composites was increased at 3 and 6 weeks after implantation into rats. Histomorphometric analysis revealed more uniform and extensive bone formation in the low pressure group than in the controls. The application of low pressure during the seeding of BMSCs in perfusing medium into a porous scaffold is useful for tissue-engineered bone formation. (c) 2005 Elsevier Ltd. All rights reserved.