Nucleoporin 88 expression in normal and neoplastic squamous epithelia of the uterine cervix
Nucleoporin 88 expression in normal and neoplastic squamous epithelia of the uterine cervix
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DOI：
10.1016/j.anndiagpath.2009.05.005
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发表时间：
2009-10-01
影响因子：
2
通讯作者：
Hager, Martina
中科院分区：
文献类型：
--
作者：
Brustmann, Hermann;Hager, Martina
本研究调查了甲醛固定、石蜡包埋的宫颈标本存档组织中核孔蛋白88（Nup 88）的表达，所述宫颈标本包括正常宫颈外鳞状上皮（n = 34）、对应于宫颈上皮内瘤变（CIN）1的低度鳞状上皮内病变（n = 9）、对应于CIN 2和CIN 3的高度鳞状上皮内病变（n = 28）、和浸润性鳞状细胞癌（ISCC; n = 30），以确定该因子的表达是否参与从正常宫颈上皮到CIN和宫颈ISCC的形态学谱的进展。使用Ventana BenchMark XT免疫染色仪和针对Nup 88的小鼠抗人单克隆抗体进行标准免疫组织化学技术。免疫染色根据阳性染色细胞的数量和强度进行评分，每种情况下的最终免疫评分为0至12。核孔蛋白88免疫评分从正常宫颈鳞状上皮到CIN 1、CIN 2/3和ISCC显著增加（P <0.0001，方差分析）。宫颈上皮内瘤样病变2/3作为孤立性病变与邻近ISCC无显著性差异，邻近ISCC和相应ISCC的CIN 2/3免疫评分显著相关（P = .0007）。这项研究表明，与正常宫颈外鳞状上皮和CIN 1相比，Nup 88在高级别CIN病变和ISCC中显著过表达。然而，Nup 88评估作为个体病例的诊断标志物的价值有限。(C)2009 Elsevier Inc. All rights reserved.
This study investigated the expression of nucleoporin 88 (Nup88) in formalin-fixed, paraffin-embedded archival tissues of cervical specimens consisting of normal ectocervical squamous epithelia (n = 34), low-grade squamous intraepithelial lesions corresponding to cervical intraepithelial neoplasia (CIN) 1 (n = 9), high-grade squamous intraepithelial lesions corresponding to CIN2 and CIN3 (n = 28), and invasive squamous cell carcinoma (ISCC; n = 30) to determine whether expression of this factor is involved in the progression of the morphological spectrum from normal cervical epithelia to CIN and cervical ISCC. A standard immunohistochemical technique was performed using a Ventana BenchMark XT immunostainer with a mouse antihuman monoclonal antibody to Nup88. Immunostaining was scored with regard to quantity and intensity of positively stained cells, with final immunoscores from 0 to 12 in each case. Nucleoporin 88 immunoscores increased significantly from normal ectocervical squamous epithelia to CIN1, CIN2/3, and ISCC (P < .0001, analysis of variance). Cervical intraepithelial neoplasia 2/3 as isolated lesions and adjacent to ISCC did not differ significantly, A significant correlation was noticed for immunoscores of CIN2/3 adjacent to ISCC and the corresponding ISCC (P = .0007). This study indicates that Nup88 is significantly overexpressed in high-grade CIN lesions and ISCC compared with normal ectocervical squamous epithelia and CIN1. However, Nup88 evaluation is of limited value as a diagnostic marker in individual cases. (C) 2009 Elsevier Inc. All rights reserved.