LEAF DISK TRANSFORMATION OF CULTIVATED TOMATO (LYCOPERSICON-ESCULENTUM) USING AGROBACTERIUM-TUMEFACIENS
LEAF DISK TRANSFORMATION OF CULTIVATED TOMATO (LYCOPERSICON-ESCULENTUM) USING AGROBACTERIUM-TUMEFACIENS
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DOI：
10.1007/bf00269239
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发表时间：
1986-01-01
影响因子：
6.2
通讯作者：
FRALEY, R
中科院分区：
文献类型：
--
作者：
MCCORMICK, S;NIEDERMEYER, J;FRALEY, R
以番茄（L. esculentum）。叶外植体和子叶/下胚轴切片均可用于再生转化植物。我们已经从8个番茄品种中获得了300多株转基因植株。我们有证据表明T-DNA的单拷贝和多拷贝插入，并证明了T-DNA插入的遗传符合预期的孟德尔比率。几种异源启动子在番茄中起作用。与共整合T-DNA载体相比，观察到二元T-DNA载体的转化效率降低。叶盘法的简易性使番茄成为植物生物技术的首要实验生物。
The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.