Genome-wide prediction and comparative transcriptomic analysis reveals the G protein-coupled receptors involved in gonadal development of Apostichopus japonicus
Genome-wide prediction and comparative transcriptomic analysis reveals the G protein-coupled receptors involved in gonadal development of Apostichopus japonicus
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全基因组预测和比较转录组分析揭示了刺参性腺发育中涉及的G蛋白偶联受体
DOI：
10.1016/j.ygeno.2020.10.030
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发表时间：
2021-01-25
期刊：
影响因子：
4.4
通讯作者：
Wang, Tianming
中科院分区：
文献类型：
--
作者：
Huang, Dexiang;Zhang, Bing;Wang, Tianming
仿刺参雌雄异株，季节性繁殖。G蛋白偶联受体（GPCR）介导的信号系统可能在A. - 是的在这里，我们通过计算机对基因组中的GPCR进行了分类，并使用转录组分析来进一步挖掘那些在性腺发育控制中发挥作用的基因。从A.其中183个被进一步注释为分子途径。转录组分析显示，327个GPCR在性腺中表达，这些被分为4个家族和19个亚家族。有三种途径与生殖有关，包括神经活性配体-受体相互作用、mTOR和Wnt信号通路。筛选出7个和8个卵巢和睾丸特异的GPCR，并利用qPCR技术检测了不同发育阶段的多个组织和性腺中的基因表达谱。这些结果为发现GPCR介导的信号调控在海参生殖特别是性腺发育调控中的作用提供了新的思路。
The sea cucumber Apostichopus japonicus is dioecious, with seasonal reproduction. G protein-coupled receptor (GPCR)-mediated signaling systems might play critical roles in the reproductive control of A. japonicus. Here, we classified GPCR from the genome in silico and used transcriptomic analyses to further mine those that function in gonadal-development control. Totally, 487 GPCRs were predicted from A. japonicus, and 183 of these were further annotated to molecular pathways. Transcriptome analysis revealed 327 GPCRs expressed in gonads, and these were classified into four families and 19 subfamilies. Three pathways were apparently associated with reproduction, including neuroactive ligand-receptor interaction, the mTOR and Wnt signaling pathways. Seven and eight ovaryand testis-specific GPCRs were filtered, and the gene expression profiles were determined in multiple tissues and gonads at different developmental stages by qPCR. These results provide new insights into the discovery of GPCR-mediated signaling control in sea cucumber reproduction, especially in gonadal development control.