Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks
Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks
复制标题
DOI：
10.1016/s0006-3495(01)75809-2
复制
发表时间：
2001-09-01
影响因子：
3.4
通讯作者：
Uyeda, TQP
中科院分区：
文献类型：
--
作者：
Hiratsuka, Y;Tada, T;Uyeda, TQP
马达蛋白能够在体外移动蛋白丝。然而，有用的工作不能从现有的体外系统中提取，因为细丝运动是在二维表面上的随机方向。我们成功地限制驱动蛋白驱动的微管沿着线性轨道的运动，通过使用微米级的凹槽光刻制造在玻璃表面上。我们还通过在轨迹上添加箭头图案，实现了从沿线性轨迹的沿着双向运动中提取单向运动。这些“整流器”使我们能够构建微型化的循环器，其中微管群体在一个方向上旋转，并积极运输微管之间的两个池连接的箭头轨道在微米尺度的领域。
Motor proteins are able to move protein filaments in vitro. However, useful work cannot be extracted from the existing in vitro systems because filament motions are in random directions on two-dimensional surfaces. We succeeded in restricting kinesin-driven movements of microtubules along linear tracks by using micrometer-scaled grooves lithographically fabricated on glass surfaces. We also accomplished the extraction of unidirectional movement from the bidirectional movements along the linear tracks by adding arrowhead patterns on the tracks. These "rectifiers" enabled us to construct microminiturized circulators in which populations of microtubules rotated in one direction, and to actively transport microtubules between two pools connected by arrowheaded tracks in the fields of micrometer scales.