Microbuckling of fibrin provides a mechanism for cell mechanosensing
Microbuckling of fibrin provides a mechanism for cell mechanosensing
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DOI：
10.1098/rsif.2015.0320
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发表时间：
2015-07-06
影响因子：
3.9
通讯作者：
Ravichandran, Guruswami
中科院分区：
文献类型：
--
作者：
Notbohm, Jacob;Lesman, Ayelet;Ravichandran, Guruswami
生物细胞感知机械力并做出反应，但这种机械传感过程如何在非线性不均匀纤维基质中发生仍然未知。我们表明，纤维基质中的细胞会引起变形场，其传播范围比线性弹性预测的范围更长。许多力传导研究中使用的合成线弹性水凝胶未能捕捉到这种效应。我们开发了纤维网络的非线性微观结构有限元模型，以模拟由细胞引起的局部变形。该模型从实验中捕获测量到的细胞引起的基质位移，并确定了远程细胞机械传感的重要机制：由于单个纤维的微屈曲而导致的压缩刚度损失。我们证明，细胞通过由这种机制引起的局部细胞间拉伸变形带的形成来相互感知。
Biological cells sense and respond to mechanical forces, but how such a mechanosensing process takes place in a nonlinear inhomogeneous fibrous matrix remains unknown. We show that cells in a fibrous matrix induce deformation fields that propagate over a longer range than predicted by linear elasticity. Synthetic, linear elastic hydrogels used in many mechanotransduction studies fail to capture this effect. We develop a nonlinear microstructural finite-element model for a fibre network to simulate localized deformations induced by cells. The model captures measured cell-induced matrix displacements from experiments and identifies an important mechanism for long-range cell mechanosensing: loss of compression stiffness owing to micro, buckling of individual fibres. We show evidence that cells sense each other through the formation of localized intercellular bands of tensile deformations caused by this mechanism.