Multiple cell populations generate macrophage progenitors in the early yolk sac
Multiple cell populations generate macrophage progenitors in the early yolk sac
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DOI：
10.1007/s00018-022-04203-7
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发表时间：
2022-03-01
影响因子：
8
通讯作者：
Yamane, Toshiyuki
中科院分区：
文献类型：
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作者：
Ito, Chie;Hikosaka-Kuniishi, Mari;Yamane, Toshiyuki
卵黄囊(YS)CSF1受体阳性(CSF1R(+))细胞被认为是存在于各种组织中的组织驻留巨噬细胞的前体细胞。组织驻留巨噬细胞的YS祖细胞被称为红系髓系祖细胞(EMPs)。然而，在早期YS中存在不同类型的造血祖细胞，因此还不能确切地知道哪种类型的造血细胞产生CSF1R(+)血统。在这项研究中，我们分析了CSF1R(+)祖细胞何时出现在YS早期。结果表明，早在胚胎第9天(E9)，YS就出现了CSF1R(+)细胞，分化为CSF1R(+)细胞的造血祖细胞最早出现在E8。由于这些祖细胞具有产生原始红系细胞的能力，原始红系血统很可能与CSF1R(+)血统具有共同的祖细胞。当CSF1R(+)细胞出现在YS早期时，PU.1和GATA1之间似乎相互拮抗。一天后(E9)，YS中的多个祖细胞，包括髓系限制祖细胞和多能祖细胞，可以立即产生CSF1R(+)细胞。这些结果表明，EMPs不是CSF1R(+)谱系的唯一来源；相反，在YS早期，多个造血细胞群体导致CSF1R(+)谱系的产生。
Yolk sac (YS) CSF1 receptor positive (CSF1R(+)) cells are thought to be the progenitors for tissue-resident macrophages present in various tissues. The YS progenitors for tissue-resident macrophages are referred to as erythroid-myeloid progenitors (EMPs). However, diverse types of hematopoietic progenitors are present in the early YS, thus it is not precisely known which type of hematopoietic cell gives rise to the CSF1R(+) lineage. In this study, an analysis was conducted to determine when CSF1R(+) progenitors appeared in the early YS. It showed that CSF1R(+) cells appeared in the YS as early as embryonic day 9 (E9) and that the earliest hematopoietic progenitors that differentiate into CSF1R(+) cells were found in E8. Since these progenitors possessed the capability to generate primitive erythroid cells, it was likely that primitive erythroid lineages shared progenitors with the CSF1R(+) lineage. Mutual antagonism appears to work between PU.1 and GATA1 when CSF1R(+) cells appear in the early YS. One day later (E9), multiple progenitors, including myeloid-restricted progenitors and multipotent progenitors, in the YS could immediately generate CSF1R(+) cells. These results suggest that EMPs are not an exclusive source for the CSF1R(+) lineage; rather, multiple hematopoietic cell populations give rise to CSF1R(+) lineage in the early YS.