STAGES OF EMBRYONIC-DEVELOPMENT OF THE ZEBRAFISH
STAGES OF EMBRYONIC-DEVELOPMENT OF THE ZEBRAFISH
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DOI：
10.1002/aja.1002030302
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发表时间：
1995-07-01
影响因子：
2.5
通讯作者：
SCHILLING, TF
中科院分区：
文献类型：
--
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
我们描述了斑马鱼胚胎发育的一系列阶段，丹尼奥（Brachydanio）版本。我们定义了胚胎发生的七个时期——合子期、卵裂期、囊胚期、原胚期、分节期、咽期和孵化期。这些分裂突出了受精后3天内发生的主要发育过程的变化谱，我们回顾了一些已知的形态发生和发生在每个时期的其他重要事件。阶段细分了时期，阶段是命名的，而不是像大多数其他系列那样编号，随着我们对这个物种的发展了解的更多，阶段系列提供了灵活性和持续的进化。这些阶段和它们的名字是基于形态学特征的，通常很容易通过解剖体视显微镜检查活胚胎来识别。该描述还充分利用了活体胚胎的光学透明性，当使用复合显微镜和诺马尔斯基干涉对比照明检查胚胎时，甚至可以看到非常深的结构。显微照片和复合相机清晰的线条图描绘了这些阶段。还有一些图形描绘了用作救助路标的独特人物的发展。(C) 1995 Wiley-Liss, Inc。
We describe a series of Stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio. We define seven broad periods of embryogenesis-the zygote, cleavage, blastula, gastrula, segmentation, pharyngula, and hatching periods. These divisions highlight the changing spectrum of major developmental processes that occur during the first 3 days after fertilization, and we review some of what is known about morphogenesis and other significant events that occur during each of the periods. Stages subdivide the periods, Stages are named, not numbered as in most other series, providing for flexibility and continued evolution of the staging series as we learn more about development in this species. The stages, and their names, are based on morphological features, generally readily identified by examination of the live embryo with the dissecting stereomicroscope. The descriptions also fully utilize the optical transparancy of the Live embryo, which provides for visibility of even very deep structures when the embryo is examined with the compound microscope and Nomarski interference contrast illumination. Photomicrographs and composite camera lucida line drawings characterize the stages pictorially. Other figures chart the development of distinctive characters used as staging aid signposts. (C) 1995 Wiley-Liss, Inc.