LEAF AND CHLOROPLAST DEVELOPMENT IN RELATION TO NUTRIENT AVAILABILITY
LEAF AND CHLOROPLAST DEVELOPMENT IN RELATION TO NUTRIENT AVAILABILITY
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DOI：
10.1016/s0176-1617(89)80145-2
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发表时间：
1989-07-01
影响因子：
4.3
通讯作者：
DIETZ, KJ
中科院分区：
文献类型：
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作者：
DIETZ, KJ
将在完全营养液上水培生长的菠菜植物转移到硫酸盐和磷酸盐缺乏的培养基中两周。在此期间之后，植物显示在硫酸盐饥饿下全植物叶面积减少76%，在磷酸盐饥饿下减少69%。收获所有叶片并根据其相对年龄分析阴离子、氨基酸、蛋白质、核酸和叶绿素含量。不同处理下老叶和幼叶无机磷、硝酸盐和硫酸盐含量不同。阴离子的分布反映了它们的相对迁移率。在所有条件下，与对照相比，幼叶中的总核酸最高，但在磷酸盐和硫酸盐缺乏下降低了40%和60%。不同叶龄叶片间蛋白质的分布与核酸的分布不同。在缺硫植株中，蛋白质含量在幼叶中最低，随叶龄的增加而增加;叶绿素含量随叶龄的增加而增加。与叶面积相关的光合CO2固定在磷和硫酸盐饥饿植物的叶片中减少。叶绿素含量与CO2固定率呈正相关。结果表明，叶片代谢的灵活性，使植物能够处理不同类型的养分缺乏所施加的压力情况。
Spinach plants grown in hydroponic culture on complete nutrient solutions were transferred to sulfate and phosphate deficient media for two weeks. After this period, the plants revealed a reduction in whole plant leaf area of 76% under sulfate starvation and 69% under phosphate starvation. All leaves were harvested and analyzed for anions, amino acids, protein, nucleic acids and chlorophyll content according to their relative age. Levels of inorganic phosphate, nitrate and sulfate were different in young versus old leaves under the various treatments. The distribution of anions reflected their relative mobility. Under all conditions total nucleic acids were highest in young leaves but reduced by 40 and 60 % under phosphate and sulfate deficiency as compared to the control. The protein distribution between leaves of various ages differed from the distribution of the nucleic acids. In sulfate deficient plants, protein content was lowest in young leaves and increased with leaf age; the chlorophyll content increased with increasing leaf age. Leaf area related photosynthetic CO2 fixation was reduced in leaves of phosphate and sulfate starved plants. There was a positive correlation between chlorophyll content and CO2 fixation rate. The results illustrate the flexibility of leaf metabolis, which enables plants to handle stress situations imposed by different kinds of nutrient deficiency.