Thrombopoietin expands erythroid, granulocyte-macrophage, and megakaryocytic progenitor cells in normal and myelosuppressed mice.
Thrombopoietin expands erythroid, granulocyte-macrophage, and megakaryocytic progenitor cells in normal and myelosuppressed mice.
复制标题
DOI：
--
复制
发表时间：
1996-02
影响因子：
2.6
通讯作者：
K. Kaushansky;N. Lin;A. Grossmann;J. Humes;K. Sprugel;V. Broudy
中科院分区：
文献类型：
--
作者：
K. Kaushansky;N. Lin;A. Grossmann;J. Humes;K. Sprugel;V. Broudy
血小板生成素（Tpo）是原癌基因受体c-Mpl的配体，可增加巨核细胞的大小、倍性和血小板特异性糖蛋白的表面表达，与血小板质量呈负相关，是体内血小板生成的有效刺激物。然而，c-mpl生物学的几个特征，以及它的病毒对应物v-mpl，表明Tpo的作用可能不严格限于巨核细胞生成。为了研究Tpo可能影响多种细胞谱系的可能性，我们研究了体内施用激素对多种类型的骨髓和脾克隆生成造血祖细胞的影响。我们报告说，Tpo的行为，以扩大BFU-E，CFU-GM，CFU-Mk和CFU-E在正常小鼠中的重新分配，并加速恢复所有这些祖细胞类型的骨髓抑制动物。这些研究结果表明，造血祖细胞隔室响应Tpo作为一个整体，Tpo管理的体内效应可能比以前预期的范围更广。
Thrombopoietin (Tpo), the ligand for the proto-oncogene receptor c-Mpl, increases megakaryocyte size, ploidy, and surface expression of platelet-specific glycoproteins, is inversely related to platelet mass, and is a potent in vivo stimulus of platelet production. However, several features of c-mpl biology, and that of its viral counterpart v-mpl, suggest that the action of Tpo may not be strictly limited to megakaryocytopoiesis. To investigate the possibility that Tpo might affect a multitude of cell lineages, we studied the effects of in vivo administration of the hormone on multiple types of marrow and splenic clonogenic hematopoietic progenitors. We report that Tpo acts to expand BFU-E, CFU-GM, and CFU-Mk and redistribute CFU-E in normal mice and to hasten the recovery of all of these progenitor cell types in myelosuppressed animals. These findings argue that the hematopoietic progenitor cell compartment responds to Tpo as a whole and that the in vivo effects of Tpo administration may be more wide-ranging than previously anticipated.