Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry
Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry
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DOI：
10.1038/nbt0498-381
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发表时间：
1998-04-01
影响因子：
46.9
通讯作者：
Köster, H
中科院分区：
文献类型：
--
作者：
Fu, DJ;Tang, K;Köster, H
基质辅助激光解吸电离飞行时间质谱法用于测序人类p53基因的外显子5至8。建立了用于靶扩增和质谱（MS）测序的单管程序。MS测序方案设计用于高通量和平行样品处理，并且适合于全自动化。使用fmol样品量获得可靠的测序数据。通过杂合模板的直接测序证明了MS测序的高分辨率和准确性。
Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.