Enzyme-Free Saccharification of Cellulose to Glucose Using Ultrasonic Welding
Enzyme-Free Saccharification of Cellulose to Glucose Using Ultrasonic Welding
复制标题
DOI：
10.1016/j.ijft.2022.100137
复制
发表时间：
2022-01
影响因子：
--
通讯作者：
S. Nomura;Taiki Murase;Takeru Kodama;S. Mukasa;Y. Iwamoto
中科院分区：
文献类型：
--
作者：
S. Nomura;Taiki Murase;Takeru Kodama;S. Mukasa;Y. Iwamoto
超声波焊接是一种通过施加微量的超声波振动和压力，可以瞬间融合和连接热塑性树脂的加工技术。本研究表明，由超声波焊接引起的摩擦力或压力可以在没有糖化酶或催化剂的情况下引起纤维素分解。使用喇叭型换能器将19.5 kHz的超声波向下照射到样品上。纤维素样品由15 - 105张堆叠的具有高纤维素含量的滤纸、一张棉和一张大麻组成。纤维素与超声波喇叭尖端直接接触产生的高摩擦力在短时间内分解葡萄糖以产生葡萄糖。纤维素在无酶环境中通过超声波照射水解分解成葡萄糖，但随后通过由连续超声波照射引起的反应场温度升高引起的脱水反应进一步分解成5-羟甲基糠醛、糠醛和其它化合物。通过超声波焊接，可以从纤维素中获得葡萄糖而不使用酶。用该方法分解纤维素、棉花和大麻的结果表明，通过分解滤纸可以获得1.2%的葡萄糖产率。
Ultrasonic welding is a processing technology that can instantly fuse and join thermoplastic resins by applying minute amounts of ultrasonic vibration and pressure. The present study demonstrates that the frictional force or pressure caused by ultrasonic welding can cause cellulose decomposition without a saccharifying enzyme or catalysts. Ultrasonic waves of 19.5 kHz were irradiated downward on samples using a horn-type transducer. The cellulose specimens were composed of 15 – 105 stacked sheets of filter paper with high cellulose content, a sheet of cotton, and a sheet of hemp. The high frictional forces resulting from direct contact of cellulose with the ultrasonic horn tips decomposed the saccharides within a short time to produce glucose. Cellulose is decomposed into glucose through hydrolysis in an enzyme-free environment by ultrasonic irradiation, but then it is further decomposed into 5-hydroxymethylfurfural, furfural, and other compounds via a dehydration reaction caused by an increase in temperature of the reaction field from continuous ultrasonic irradiation. Glucose could be obtained from the cellulose without using an enzyme through ultrasonic welding. The result of decomposing cellulose, cotton, and hemp by this method revealed that a yield of 1.2% glucose could be obtained by decomposing filter paper.