AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development
AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development
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DOI：
10.1093/pcp/pcz235
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发表时间：
2020-04-01
影响因子：
4.9
通讯作者：
Chiba, Yukako
中科院分区：
文献类型：
--
作者：
Motomura, Kazuki;Arae, Toshihiro;Chiba, Yukako
开花植物雄配子体花粉的发育受基因表达动态变化的严格控制。最近的研究，以澄清花粉发育的分子方面揭示了参与诱导基因表达的几个转录因子。然而，有限的信息是参与基因表达的负调控，以消除不必要的转录在花粉发育过程中的因素。在这项研究中，我们发现AtNOT 1是一个必要的蛋白质适当的花粉发育和萌发能力。AtNOT 1是AtCCR 4-NOT复合体的一个支架蛋白，在拟南芥中包含多个与mRNA转录调控相关的组分。atnot 1杂合子突变体花粉的表型分析表明，成熟的突变体花粉不能萌发，也揭示了异常定位的核和一个特定的蛋白质在三细胞花粉阶段。此外，atnot 1杂合子突变体花粉的转录组分析表明，大量的转录本的下调，沿着的特定转录本的上调所需的花粉管萌发的AtNOT 1在后期microgametogenesis，是重要的适当的花粉发育和萌发。总的来说，我们的研究结果提供了新的见解花粉发育过程中的基因表达的负调控，通过显示atnot 1杂合子突变花粉的严重缺陷的表型。
Development of pollen, the male gametophyte of flowering plants, is tightly controlled by dynamic changes in gene expression. Recent research to clarify the molecular aspects of pollen development has revealed the involvement of several transcription factors in the induction of gene expression. However, limited information is available about the factors involved in the negative regulation of gene expression to eliminate unnecessary transcripts during pollen development. In this study, we revealed that AtNOT1 is an essential protein for proper pollen development and germination capacity. AtNOT1 is a scaffold protein of the AtCCR4-NOT complex, which includes multiple components related to mRNA turnover control in Arabidopsis. Phenotypic analysis using atnot1 heterozygote mutant pollen showed that the mature mutant pollen failed to germinate and also revealed abnormal localization of nuclei and a specific protein at the tricellular pollen stage. Furthermore, transcriptome analysis of atnot1 heterozygote mutant pollen showed that the downregulation of a large number of transcripts, along with the upregulation of specific transcripts required for pollen tube germination by AtNOT1 during late microgametogenesis, is important for proper pollen development and germination. Overall, our findings provide new insights into the negative regulation of gene expression during pollen development, by showing the severely defective phonotype of atnot1 heterozygote mutant pollen.