Characterization of biological effect of 1763 MHz radiofrequency exposure on auditory hair cells
Characterization of biological effect of 1763 MHz radiofrequency exposure on auditory hair cells
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DOI：
10.1080/09553000802460123
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Park, Woong-Yang
中科院分区：
文献类型：
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作者：
Huang, Tai-Qin;Lee, Min Su;Park, Woong-Yang
目的：据报告，在体外和体内模型中，移动的电话频率的辐射（RF）暴露不会引起细胞损伤。我们选择HEI-OC 1永生化小鼠听觉毛细胞来表征细胞对1763 MHz RF暴露的反应，因为听觉细胞可以暴露于移动的电话频率。材料与方法：将细胞在码分多址（CDMA）暴露室中以20 W/kg比吸收率（SAR）暴露于1763 MHz RF 24和48 h，以检查细胞周期、DNA损伤、应激反应和基因表达的变化。结果：RF暴露后，细胞周期无明显改变，DNA无明显损伤。热休克蛋白（HSP）的表达和丝裂原活化蛋白激酶（MAPK）的磷酸化也没有改变。我们试图用微阵列鉴定基因表达的任何改变。使用应用生物系统1700全基因组表达小鼠微阵列，我们发现，只有29个基因（0.09%的总基因检查）的变化超过1.5倍的RF暴露。结论：从这些结果中，我们没有发现任何证据表明，在1763 MHz的RF暴露后，在SAR为20 W/kg的HEI-OC 1听毛细胞，包括细胞周期分布，DNA损伤，应激反应和基因表达的诱导。
Purpose: Radiofrequency (RF) exposure at the frequency of mobile phones has been reported not to induce cellular damage in invitro and invivo models. We chose HEI-OC1 immortalized mouse auditory hair cells to characterize the cellular response to 1763 MHz RF exposure, because auditory cells could be exposed to mobile phone frequencies. Materials and methods: Cells were exposed to 1763 MHz RF at a 20W/kg specific absorption rate (SAR) in a code division multiple access (CDMA) exposure chamber for 24 and 48h to check for changes in cell cycle, DNA damage, stress response, and gene expression. Results: Neither of cell cycle changes nor DNA damage was detected in RF-exposed cells. The expression of heat shock proteins (HSP) and the phosphorylation of mitogen-activated protein kinases (MAPK) did not change, either. We tried to identify any alteration in gene expression using microarrays. Using the Applied Biosystems 1700 full genome expression mouse microarray, we found that only 29 genes (0.09% of total genes examined) were changed by more than 1.5-fold on RF exposure. Conclusion: From these results, we could not find any evidence of the induction of cellular responses, including cell cycle distribution, DNA damage, stress response and gene expression, after 1763 MHz RF exposure at an SAR of 20W/kg in HEI-OC1 auditory hair cells.