An interplay between bZIP16, bZIP68, and GBF1 regulates nuclear photosynthetic genes during photomorphogenesis in Arabidopsis.
An interplay between bZIP16, bZIP68, and GBF1 regulates nuclear photosynthetic genes during photomorphogenesis in Arabidopsis.
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DOI：
10.1111/nph.19219
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发表时间：
2023-08
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影响因子：
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通讯作者：
Louise Norén Lindbäck;Yan Ji;Luis Cervela-Cardona;Xu Jin;Ullas V. Pedmale;Åsa Strand
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文献类型：
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作者：
Louise Norén Lindbäck;Yan Ji;Luis Cervela-Cardona;Xu Jin;Ullas V. Pedmale;Åsa Strand
幼苗发育成具有光合作用活性的植物是一个至关重要的过程。尽管它很重要，但我们并不完全了解功能性叶绿体建立背后的调控机制。我们在此通过鉴定三个基本区域/亮氨酸拉链（bZIP）转录因子：bZIP16、bZIP68和GBF1的功能，为早期光反应提供了新的见解。这些蛋白质参与调节建立光合活性叶绿体所需的关键成分。这些 bZIP 的活性取决于保守半胱氨酸残基的氧化还原状态，这提供了微调光响应基因表达的机制。蓝光隐花色素 (CRY) 光感受器提供了主要的光信号通路之一，bZIP 靶基因与三分之一的 CRY 调节基因重叠，并富集了光合作用/叶绿体相关基因。 bZIP16、bZIP68 和 GBF1 被证明是 CRY1 的新型相互作用伙伴。 CRY1 和 bZIP16 之间的相互作用受到蓝光的刺激。此外，我们证明了bZIP蛋白和隐花色素之间的遗传联系，因为cry1cry2突变体是cry1cry2bzip16bzip68gbf1突变体的上位性。 bZIP16、bZIP68 和 GBF1 调节光合作用相关基因的子集，以响应蓝光，这对于拟南芥的正常绿化过程至关重要。
The development of a seedling into a photosynthetically active plant is a crucial process. Despite its importance, we do not fully understand the regulatory mechanisms behind the establishment of functional chloroplasts. We herein provide new insight into the early light response by identifying the function of three basic region/leucine zipper (bZIP) transcription factors: bZIP16, bZIP68, and GBF1. These proteins are involved in the regulation of key components required for the establishment of photosynthetically active chloroplasts. The activity of these bZIPs is dependent on the redox status of a conserved cysteine residue, which provides a mechanism to finetune light-responsive gene expression. The blue light cryptochrome (CRY) photoreceptors provide one of the major light-signaling pathways, and bZIP target genes overlap with one-third of CRY-regulated genes with an enrichment for photosynthesis/chloroplast-associated genes. bZIP16, bZIP68, and GBF1 were demonstrated as novel interaction partners of CRY1. The interaction between CRY1 and bZIP16 was stimulated by blue light. Furthermore, we demonstrate a genetic link between the bZIP proteins and cryptochromes as the cry1cry2 mutant is epistatic to the cry1cry2bzip16bzip68gbf1 mutant. bZIP16, bZIP68, and GBF1 regulate a subset of photosynthesis associated genes in response to blue light critical for a proper greening process in Arabidopsis.