Geraniin down regulates gamma radiation-induced apoptosis by suppressing DNA damage
Geraniin down regulates gamma radiation-induced apoptosis by suppressing DNA damage
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DOI：
10.1016/j.fct.2013.03.022
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Jee, Youngheun
中科院分区：
文献类型：
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作者：
Bing, So Jin;Ha, Danbee;Jee, Youngheun
伽马射线照射引发DNA损伤和增殖干细胞和外周免疫细胞的凋亡，导致肠隐窝和淋巴系统的破坏。天竺葵苷是从水生植物龙花百合中提取的天然化合物，具有良好的抗氧化性能。在这项研究中，我们证明了天竺葵蛋白可以拯救放射敏感的脾细胞和空肠隐窝细胞免受辐射诱导的DNA损伤和凋亡。天竺葵苷处理的C57BL/6小鼠脾细胞对2gy辐射损伤的保护作用是通过增强细胞增殖和抑制DNA损伤来实现的。此外，天竺葵素通过降低促凋亡p53和Bax的表达水平和免疫反应性，提高抗凋亡Bcl-2的表达水平和免疫反应性，抑制放射敏感脾细胞的凋亡。在暴露于辐射的小鼠中，天竺葵素治疗可保护脾细胞和肠隐窝细胞免受辐射诱导的细胞死亡。我们的研究结果表明，天竺葵蛋白通过调节脾细胞DNA损伤、发挥免疫刺激能力和抑制放射敏感免疫细胞和空肠隐窝细胞凋亡来发挥辐射保护作用。因此，天竺葵素可作为抗7-辐照的辐射防护剂。(C) 2013 Elsevier Ltd.版权所有。
Gamma ray irradiation triggers DNA damage and apoptosis of proliferating stem cells and peripheral immune cells, resulting in the destruction of intestinal crypts and lymphoid system. Geraniin is a natural compound extracts from an aquatic plant Nymphaea tetragona and possesses good antioxidant property. In this study, we demonstrate that geraniin rescues radiosensitive splenocytes and jejunal crypt cells from radiation-induced DNA damage and apoptosis. Isolated splenocytes from C57BL/6 mice treated with geraniin were protected against radiation injury of 2 Gy irradiation through the enhancement of the proliferation and attenuation of DNA damage. Also, geraniin inhibited apoptosis in radiosensitive splenocytes by reducing the expression level and immunoreactivity of proapoptotic p53 and Bax and increasing those of anti-apoptotic Bcl-2. In mice exposed to radiation, geraniin treatment protected splenocytes and intestinal crypt cells from radiation-induced cell death. Our results suggest that geraniin presents radioprotective effects by regulating DNA damage on splenocytes, exerting immunostimulatory capacities and inhibiting apoptosis of radiosensitive immune cells and jejunal crypt cells. Therefore, geraniin can be a radioprotective agent against 7-irradiation exposure. (C) 2013 Elsevier Ltd. All rights reserved.