Protective effects of resveratrol against streptozotocin-induced diabetes in rats by modulation of visfatin/sirtuin-1 pathway and glucose transporters
Protective effects of resveratrol against streptozotocin-induced diabetes in rats by modulation of visfatin/sirtuin-1 pathway and glucose transporters
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DOI：
10.3109/09637486.2014.1003534
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发表时间：
2015-05-01
影响因子：
3.9
通讯作者：
Sahin, Kazim
中科院分区：
文献类型：
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作者：
Gencoglu, Hasan;Tuzcu, Mehmet;Sahin, Kazim
本实验旨在阐明白藜芦醇对糖尿病的保护作用。40只雄性Wistar大鼠随机分为4组：正常对照组和链脲佐菌素(STZ)诱导的糖尿病组，分别给予安慰剂(1ml/kg，ip)。或白藜芦醇(20 mg/kg，i.p.)持续8周。监测体重、血糖和血清丙二醛(MDA)浓度。实验结束后，用免疫印迹法检测内脂素、sirtuin-1(SIRT1)和葡萄糖转运蛋白(Gluts，2和4)在骨骼肌和胰腺的表达水平。白藜芦醇治疗部分补偿了体重减轻和高血糖，并使血清丙二醛浓度恢复到对照组水平。数据表明，补充剂可能通过抑制氧化应激和增加肝外组织内化葡萄糖的可能性来降低糖尿病及其并发症的严重程度。
This experiment was aimed at elucidating the protective effect of resveratrol against diabetes. Forty male Wistar albino rats were allocated into four groups: the control and streptozotocin (STZ)-induced diabetes groups were treated either with placebo (1 ml/kg, i.p.) or resveratrol (20 mg/kg, i.p.) for 8 weeks. Body weight, blood glucose and serum malondialdehyde (MDA) concentrations were monitored. At the end of the experimental period, expression levels of visfatin, sirtuin-1 (SIRT1) and glucose transporters (GLUTs, 2 and 4) were measured in skeletal muscle and pancreas by Western blotting. The resveratrol treatment partially compensated for body weight loss and alleviated hyperglycaemia and returned serum MDA concentrations to the control group levels. Data suggest that supplementation may reduce the severity of diabetes and its complications through suppressing oxidative stress and increasing potential to internalise glucose by extrahepatic tissues.