Effect of peroxisome proliferators on intrachromosomal and interchromosomal recombination in yeast.
Effect of peroxisome proliferators on intrachromosomal and interchromosomal recombination in yeast.
复制标题
过氧化物酶体增殖剂对酵母染色体内和染色体间重组的影响。
DOI：
10.1093/carcin/11.1.173
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发表时间：
1990
期刊：
影响因子：
4.7
通讯作者：
Reddy,JK
中科院分区：
文献类型：
--
作者：
Schiestl,RH;Reddy,JK
过氧化物酶体增殖物是一类非致突变的肝癌致癌物，可引起类似的多效性反应，如肝肿大、肝细胞内过氧化物体的增殖和肝癌的发生。Ames试验和其他各种短期试验都不能检测到过氧化物酶增殖物。最近，酵母染色体内重组系统(DEL)已被证明可被多种非致突变致癌物诱导。其中包括许多Ames化验或各种其他短期测试无法检测到的致癌物质。在本研究中，过氧化体增殖剂[4-chloro-6-(2，3-xylidino)-2-pyrimidinyl-thio]acetic酸(WY-14,643)、methyl-2-[4-(p-chlorophenyl)phenoxy]2-methyl-propionate(氯氰菊酯甲酯)、2-methyl-2[p-(1，2，3，4-tetrahydro-1-naphthyl)phenoxy]-propionic酸(奈非诺平)、2-[4-(2，2-dichlorocyclopropyl)-phenoxy]2-methyl-propionic酸(环丙贝特)、[4-chloro-6-(2，3-xylidino)-2-pyrimidinylthio(N-βhydroxyethyl)-acetamide](BR-931)；和对氯苯氧基异丁酸乙酯(α-p-氯苯氧基异丁酸乙酯)在酵母中诱导DEL和染色体间重组的潜力已被测试。在存在或不存在大鼠肝匀浆上清液(S9)的情况下，没有发现诱导这两个系统之一的证据。这些数据支持这样一种观点，即过氧化物酶增殖物是真正的非致突变致癌物。
Peroxisome proliferators are a class of non-mutagenic hepatocarcinogens, which induce a simiar pleiotropic response such as hepatomegaly, proliferation of the peroxisomes in hepatocytes and hepatocarcinogenesis. Peroxisome proliferators are not detectable by the Ames assay and various other short-term tests. Recently a system for intrachromosomal recombination in yeast (DEL) has been shown to be inducible by a variety of non-mutagenic carcinogens. These include many carcinogens that are not detectable by the Ames assay or by various other short-term tests. In the present study the peroxisome proliferators [4-chloro-6-(2,3-xylidino)-2-pyrimidinyl-thio]acetic acid (Wy-14,643); methyl-2-[4-(p-chlorophenyl)phenoxy]2-methyl-propionate (methyl clofenopate); 2-methyl-2[p-(1,2,3,4-tetrahydro-1-naphthyl)phenoxy]-propionic acid (nafenopin); 2-[4-(2,2-dichlorocyclopropyl)-phenoxy]2-methyl-propionic acid (ciprofibrate); [4-chloro-6-(2,3-xylidino)-2-pyrimidinylthio(N-βhydroxyethyl)-acetamide](BR-931); and ethylα-p-chlorophenoxyisobutyrate (clofibrate) have been tested for their potential to induce DEL as well as interchromosomal recombination in yeast. No evidence for induction of either system has been found in the presence or the absence of the supernatant (S9) from rat liver homogenate. The data sup port the notion that peroxisome proliferators are truly nonmutagenic carcinogens.