Genetic diversity of Francisella tularensis subspecies holarctica strains isolated in Japan
Genetic diversity of Francisella tularensis subspecies holarctica strains isolated in Japan
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DOI：
10.1111/j.1348-0421.2008.00036.x
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发表时间：
2008-05-01
影响因子：
2.6
通讯作者：
Yamada, Akio
中科院分区：
文献类型：
--
作者：
Fujita, Osamu;Uda, Akihiko;Yamada, Akio
最近开发的MLVA具有很高的区分能力，个别菌株或分离株的土拉弗朗西斯菌的分型。本研究应用MLVA对33只日本F. tularensis亚种holarctica菌株的遗传多样性进行了研究。土拉菌分离。在分析的7个VNTR基因座中，Ft-M2、Ft-M10和Ft-M20基因座在日本株中表现出较高的遗传多态性，而Ft-M3在非日本株中变异最大。这些结果提供了新的扩展信息的基因组多样性之间的F。土拉热亚种holarctica分布在日本，并能够确定是否一个给定的隔离是本土的日本，通过检查这些基因座使用MLVA。
The recently developed MLVA has high discriminatory power for the typing of individual strains or isolates of Francisella tularensis. In the present study, MLVA was applied to 33 Japanese F. tularensis subspecies holarctica strains to examine the genetic diversity of F. tularensis isolated. Among the seven VNTR loci analyzed, Ft-M2, Ft-M10, and Ft-M20 loci showed high genetic polymorphism in Japanese strains, whereas Ft-M3 was most variable in non-Japanese strains. These results provide novel extended information about the genomic diversity among the strains of F. tularensis ssp. holarctica distributed in Japan and enable determination of whether a given isolate is indigenous to Japan by examining these loci using MLVA.