Male infertility testing: reactive oxygen species and antioxidant capacity
Male infertility testing: reactive oxygen species and antioxidant capacity
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DOI：
10.1016/j.fertnstert.2014.10.020
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发表时间：
2014-12-01
影响因子：
6.7
通讯作者：
Agarwal, Ashok
中科院分区：
文献类型：
--
作者：
Ko, Edmund Y.;Sabanegh, Edmund S., Jr.;Agarwal, Ashok
活性氧（ROS）是精子发育生理、获能和功能的组成部分。由感染或炎症等过程引起的ROS水平升高，可能与精子发育、功能和生育能力的畸变有关。本文综述了活性氧对精子生理学的影响、其在不孕症评估中的地位、对生殖结局的影响以及抗氧化治疗。我们对过去30年的PubMed文献进行了系统回顾，重点关注ROS和氧化应激（OS）的生理学和病因学，ROS和抗氧化剂的评价。ROS通常在生理上产生，并用于维持细胞过程，如精子成熟、获能和精子-卵母细胞相互作用。当ROS的产生超过抗氧化剂的缓冲能力时，OS就会发生，并对精子和生育能力产生负面影响。活性氧和抗氧化能力测试可以潜在地增加额外的预后信息，标准的实验室检测不育男性，虽然其作为标准的一部分，评估的作用还有待确定。ROS水平升高与精液参数异常和男性不育有关，但ROS对受精率和妊娠的影响存在争议。这部分是因为对什么类型的患者可能适合ROS测试和测定标准化缺乏共识。目前不推荐对不育男性进行常规ROS检测。（C）2014年美国生殖医学会。
Reactive oxygen species (ROS) are an integral component of sperm developmental physiology, capacitation, and function. Elevated ROS levels, from processes such as infection or inflammation, can be associated with aberrations of sperm development, function, and fertilizing capacity. We review the impact of ROS on sperm physiology, its place in infertility evaluation, the implications for reproductive outcomes, and antioxidant therapy. Our systematic review of PubMed literature from the last 3 decades focuses on the physiology and etiology of ROS and oxidative stress (OS), evaluation of ROS, and antioxidants. ROS is normally produced physiologically and is used to maintain cellular processes such as sperm maturation, capacitation, and sperm-oocyte interaction. When ROS production exceeds the buffering capacity of antioxidants, OS occurs and can have a negative impact on sperm and fertility. ROS and antioxidant capacity testing can potentially add additional prognostic information to standard laboratory testing for the infertile male, although its role as standard part of an evaluation has yet to be determined. Elevated ROS levels have been implicated with abnormal semen parameters and male infertility, but the impact of ROS on fertilization rates and pregnancy is controversial. This is partly because of the lack of consensus on what type of patients may be suitable for ROS testing and assay standardization. Routine ROS testing for the infertile male is not currently recommended. (C)2014 by American Society for Reproductive Medicine.