In vitro identification of Rns-regulated genes.

In vitro identification of Rns-regulated genes.
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Rns 调控基因的体外鉴定。

DOI:
10.1128/jb.184.4.1196-1199.2002
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发表时间:
2002
影响因子:
3.2
通讯作者:
Scott,JuneR
Scott,JuneR
中科院分区:
生物学3区
文献类型:
--
作者:
Munson,GeorgeP;Holcomb,LisaG;Alexander,HeatherL;Scott,JuneR

文献摘要

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To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenicEscherichia coligenomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame ofyiiS, which lies immediately downstream of one MBP-Rns-bound fragment.