A corn-based delivery system for animal vaccines: an oral transmissible gastroenteritis virus vaccine boosts lactogenic immunity in swine
A corn-based delivery system for animal vaccines: an oral transmissible gastroenteritis virus vaccine boosts lactogenic immunity in swine
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DOI：
10.1016/j.vaccine.2003.11.066
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发表时间：
2004-06-23
期刊：
影响因子：
5.5
通讯作者：
Streatfield, SJ
中科院分区：
文献类型：
--
作者：
Lamphear, BJ;Jilka, JM;Streatfield, SJ
重组植物表达系统提供了一种生产大量用于亚单位疫苗的选定抗原的方法。谷物是特别适合的表达载体，因为表达的蛋白质可以以相对高的浓度保存较长时间而不会降解，并且干种子可以配制成适合商业应用的口服疫苗。一种针对猪传染性胃肠炎病毒并在玉米种子中表达的候选亚单位疫苗已开发出来，用于给猪口服给药。在这里，我们证明，当对先前致敏的后备母猪施用这种疫苗时，可以提高动物血清、初乳和乳汁中的中和抗体水平。因此，这种候选疫苗可有效增强泌乳免疫力，适合在商业化之前进行大规模现场试验。 (C) 2004 Elsevier Ltd. 保留所有权利。
Recombinant plant expression systems offer a means to produce large quantities of selected antigens for subunit vaccines. Cereals are particularly well-suited expression vehicles since the expressed proteins can be stored at relatively high concentrations for extended periods of time without degradation and dry seed can be formulated into oral vaccines suitable for commercial applications. A subunit vaccine candidate directed against porcine transmissible gastroenteritis virus and expressed in corn seed has been developed for oral delivery to swine. Here, we show that this vaccine, when administered to previously sensitized gilts, can boost neutralizing antibody levels in the animals' serum, colostrum and milk. Thus, this vaccine candidate is effective at boosting lactogenic immunity and is appropriate to pursue through large-scale field trials preceding commercialization. (C) 2004 Elsevier Ltd. All rights reserved.