Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy
Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy
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DOI：
10.1073/pnas.0731953100
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发表时间：
2003-06-10
影响因子：
11.1
通讯作者：
Webb, WW
中科院分区：
文献类型：
--
作者：
Dombeck, DA;Kasischke, KA;Webb, WW
微管（MT）系综极性是神经元过程的结构和功能的诊断决定因素。在这里，极化MT结构选择性成像与二次谐波产生（SHG）显微镜在原生脑组织。发现这种SHG与脑切片和培养的神经元中的轴突共定位。由于SHG只产生于非反转对称结构，轴突MT的均匀极性导致观察到的信号，而树突中的混合极性导致相消干涉。二次谐波成像提供了一种工具，以调查动力学和功能的MT合奏极性在动态的天然脑组织结构和其他亚细胞运动结构的基础上极化MT。
Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.