Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles
Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles
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DOI：
10.1016/s0378-5173(02)00267-3
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发表时间：
2002-08-28
影响因子：
5.8
通讯作者：
Maitra, A
中科院分区：
文献类型：
--
作者：
Banerjee, T;Mitra, S;Maitra, A
在AOT/正己烷反胶束体系中制备了戊二醛交联壳聚糖纳米粒子。FTIR数据证实了聚合物网络中的交联性。由于这些颗粒的粘附性，QELS测量的它们的大小取决于水溶液中的颗粒密度。还发现颗粒大小随交联量的不同而不同。这些具有特定交联度的壳聚糖纳米颗粒的实际颗粒尺寸是在水中颗粒无限稀释时测定的。当聚合链上10%的胺基团已交联时，无限稀释时粒径约为30 nm，当所有胺基团均交联时(100%交联度)，其粒径可达110 nm。透射电子显微镜照片显示，这些颗粒呈球形，并以聚集的形式存在。静脉注射后这些微粒在小鼠体内的生物分布表明，这些微粒很容易避开RES系统，并在血液中停留相当长的一段时间。兔注射~(99m)Tm标记壳聚糖纳米粒后的伽马图像也证实了上述观察结果，因为即使在2小时后也能很容易地看到血池。伽玛图像显示了粒子在心脏、肝脏、肾脏、膀胱和脊柱中的分布。有趣的是，壳聚糖纳米颗粒的生物分布研究表明，这些颗粒也分布在骨髓中，这意味着这些纳米颗粒有可能用于骨成像和靶向目的。(C)2002年，爱思唯尔科学公司出版。
Chitosan nanoparticles cross-linked with glutaraldehyde have been prepared in AOT/n hexane reverse micellar system. The cross-linking in the polymeric network has been confirmed from FTIR data. Because of the adhesive nature of these particles, their sizes, as measured by QELS, have been found dependent on the particle density in aqueous buffer. The particle size has also been found to vary with the amount of cross-linking. The actual particle size of these chitosan nanoparticles with a particular degree of cross-linking has been determined at infinite dilution of particles in water. The particle size at infinite dilution is approximate to 30 nm diameter, when 10% of the amine groups in the polymeric chains have been cross-linked and it shoots up to 110 nm diameter when all the amine groups are cross-linked (100% cross-linked). TEM pictures show that these particles are spherical in shape and remain in the form of aggregation. The biodistribution of these particles after intravenous injections in mice showed that these particles readily evade the RES system and remain in the blood for a considerable amount of time. The gamma image of the rabbit after administration of (99m)Technetium (Tc-99m) tagged chitosan nanoparticles also confirms the above observation, as the blood pool is readily visible even after 2 h. The gamma picture shows distribution of particles in the heart, liver, kidneys, bladder and the vertebral column. Interestingly, the biodistribution studies of the chitosan nanoparticles have indicated that these particles are distributed in the bone marrow also, implying the possibility of using these nanoparticles for bone imaging and targeting purpose. (C) 2002 Published by Elsevier Science B.V.