The actin-based nanomachine at the leading edge of migrating cells
The actin-based nanomachine at the leading edge of migrating cells
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DOI：
10.1016/s0006-3495(99)77018-9
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Lanni, F
中科院分区：
文献类型：
--
作者：
Abraham, VC;Krishnamurthi, V;Lanni, F
用驻波荧光显微镜测定了移行成纤维细胞高动态超薄膜脂的两个基本参数：活细胞厚度(176+/-14 nm)和F-肌动蛋白密度(1580+/-613微米F-肌动蛋白/微米(3))。结合以往研究的数据，我们计算了肌动蛋白边缘生长的肌动蛋白细丝的密度(241+/-100/亩m)和通过肌动蛋白组装所能获得的最大力(1.86+/-0.83nN/亩m)和压力(10.5+/-4.8kpa)。我们使用了细胞变形性测量(Erickson，1980)。J.细胞科学。44：187-200；Petersen等人，1982。程序娜塔莉。阿卡德。SCI。美国。79：5327-5331)，以及对阻止单丝聚合所需的力的估计(Hill，1981)。程序纳特尔，阿卡德。SCI。美国。78：5613-5617；Peskin等人，1993，BiPhys，J.65：316-324)论证肌动蛋白组装本身可以直接驱动板脂延伸。
Two fundamental parameters of the highly dynamic, ultrathin lamellipodia of migrating fibroblasts have been determined-its thickness in living cells (176 +/- 14 nm), by standing-wave fluorescence microscopy, and its F-actin density (1580 +/- 613 mu m of F-actin/mu m(3)), via image-based photometry. In combination with data from previous studies, we have computed the density of growing actin filament ends at the lamellipodium margin (241 +/- 100/mu m) and the maximum force (1.86 +/- 0.83 nN/mu m) and pressure (10.5 +/- 4.8 kPa) obtainable via actin assembly. We have used cell deformability measurements (Erickson, 1980. J. Cell Sci. 44:187-200; Petersen et al., 1982. Proc. Natl. Acad. Sci. USA. 79:5327-5331) and an estimate of the force required to stall the polymerization of a single filament (Hill, 1981. Proc. Natl, Acad. Sci. USA. 78:5613-5617; Peskin et al., 1993, Biophys, J. 65:316-324) to argue that actin assembly alone could drive lamellipodial extension directly.