First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains
First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains
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中国沿海首次记录 Biecheleria cincta（甲藻纲），并对其菌株进行形态和分子表征
DOI：
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发表时间：
2013
影响因子：
--
通讯作者：
H. Gu
中科院分区：
文献类型：
--
作者：
Zhaohe Luo;Wei;Bin Xu;H. Gu
本文首次报道了中国沿海存在的五斑双螯蟹（Biecheleria cincta，= Woloszynskia cincta）。在扫描电镜下，上锥体中可见3 ~ 5个小泡和一个细长的顶小泡（EAV），下锥体和扣带中各有3个小泡。薄切片显示B. cincta具有柄核和一个不寻常的眼斑组成的一堆池砖状物质（E型），从而支持其转移从Woloszynskia Biecheleria。在培养中自发形成棘囊，包囊形成率约为20%。测定了中国沿海12个菌株的大亚基核糖体DNA（LSU rDNA）和内转录间隔区（ITS）序列。基于LSU rDNA和ITS序列的系统发育分析使用贝叶斯推断和最大似然法揭示了两个不同的核糖体型（称为核糖体型A和B）在B。辛克塔ITS区域在B内的成对距离。带孔的数量在0.024 ~ 0.072之间，表明存在一个复杂的隐蔽种。
The presence of Biecheleria cincta (= Woloszynskia cincta) in the Chinese coasts is reported for the first time. In scanning electron microscope, three to five series of vesicles and an elongated apical vesicle (EAV) were visible in the epicone, and both the hypocone and the cingulum had three series of vesicles each. Thin sections revealed that B. cincta possesses stalked pyrenoids and an unusual eyespot consisting of a stack of cisternae with brick-like materials (type E), thus supporting its transfer from Woloszynskia to Biecheleria. Spiny cysts formed spontaneously in culture, with an encystment rate of around 20%. Both large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer region (ITS) sequences in 12 strains from the Chinese coasts were determined. Phylogenetic analyses based on LSU rDNA and ITS sequences using Bayesian inference and maximum likelihood revealed two distinct ribotypes (referred to as ribotype A and B) in B. cincta. ITS region pairwise distances within B. cincta ranged from 0.024 to 0.072, suggesting the existence of a complex of cryptic species.
DOI：
10.1016/j.dsr2.2009.09.005
发表时间：
2010-02
影响因子：
3
作者：
Brosnahan, Michael L.;Kulis, David M.;Solow, Andrew R.;Erdner, Deana L.;Percy, Linda;Lewis, Jane;Anderson, Donald M.
通讯作者：
Anderson, Donald M.