Integration of Metabolomics and Transcriptomics Reveals the Therapeutic Mechanism Underlying Paeoniflorin for the Treatment of Allergic Asthma
Integration of Metabolomics and Transcriptomics Reveals the Therapeutic Mechanism Underlying Paeoniflorin for the Treatment of Allergic Asthma
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代谢组学和转录组学的整合揭示了芍药苷治疗过敏性哮喘的治疗机制
DOI：
10.3389/fphar.2018.01531
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发表时间：
2019-01-18
影响因子：
5.6
通讯作者：
Cao, Gang
中科院分区：
文献类型：
--
作者：
Shou, Qiyang;Jin, Lu;Cao, Gang
目的：哮喘是一种以气道重塑、高反应性和呼吸短促为特征的慢性气道炎症性疾病。芍药苷是芍药的主要活性成分之一，对多种疾病具有抗炎和免疫调节作用。然而，目前尚不清楚芍药苷治疗是否能抑制过敏性哮喘。方法：观察芍药苷对哮喘小鼠肺功能及气道炎症的影响。这些哮喘Balb/c小鼠首先通过卵清蛋白（OVA）致敏并构建。随后，我们通过整合转录组学和代谢组学数据集确定了芍药苷治疗过敏性哮喘的作用机制。结果：我们的研究结果表明，芍药苷处理小鼠的许多基因和代谢物受到调节。此外，芍药苷的潜在靶蛋白在脂肪酸代谢、炎症反应、氧化应激和局部粘附中发挥重要作用。结论：芍药苷对哮喘具有有益作用，其作用机制可能在系统水平上通过调节脂肪酸代谢、炎症反应和粘附途径实现。
Objectives: Asthma is a chronic airway inflammatory disease, which is characterized by airway remodeling, hyperreactivity and shortness of breath. Paeoniflorin is one of the major active ingredients in Chinese peony, which exerts anti-inflammatory and immune-regulatory effects in multiple diseases. However, it remains unclear whether paeoniflorin treatment can suppress allergic asthma. Methods: In this study, we evaluated the effect of paeoniflorin on lung function and airway inflammation in asthmatic mice. These asthmatic Balb/c mice were first sensitized and constructed through ovalbumin (OVA) motivation. Subsequently, we determined the mechanism of action of paeoniflorin in treating allergic asthma through integrated transcriptomic and metabolomic data sets. Results: Our results demonstrated that many genes and metabolites were regulated in the paeoniflorin-treated mice. Moreover, the potential target proteins of paeoniflorin played important roles in fatty acid metabolism, inflammatory response, oxidative stress and local adhesion. Conclusion: Paeoniflorin has a beneficial effect on asthma, which may be achieved through regulating fatty acid metabolism, inflammatory response and the adhesion pathway at system level.