Distribution of metallothionein in normal and pathological human skin.
Distribution of metallothionein in normal and pathological human skin.
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金属硫蛋白在正常和病理人类皮肤中的分布。
DOI：
10.1007/bf00375845
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发表时间：
1994
影响因子：
3
通讯作者：
M. de Ley
中科院分区：
文献类型：
--
作者：
J. J. van den Oord;M. de Ley
应用抗人胎肝金属硫蛋白（MT）单克隆抗体L2 E3研究了MT在正常和病理皮肤冰冻切片中的表达和分布。正常胎儿和成人皮肤的免疫组化染色显示，在表皮和外毛根鞘，毛基质细胞和分泌线圈的基底角质形成细胞的强烈反应，但不是外分泌部分的外分泌腺;顶泌汗腺周围的肌上皮细胞同样染色。在表皮增生，变量数量的基底上角化细胞染色，而在界面皮炎，中断染色被发现在基底层。在鳞状细胞肿瘤中观察到弱或分散的染色，而基底细胞癌没有显示出一致的染色。MT在正常皮肤中的分布与基底角质形成细胞和毛基质细胞的萌发作用一致，而其在增生表皮中的分布与实验动物数据一致，并反映了在这些条件下萌发池的增加。它的结论是，单克隆抗体L2 E3可以作为一个有价值的免疫组化标记在诊断皮肤病理学，因为它的标签选择性基底角质形成细胞，因为它区分外分泌和顶浆汗腺。
The expression and distribution of metallothionein (MT) in frozen sections of normal and pathological human skin was studied using the monoclonal antibody L2E3 directed against MT derived from human fetal liver. Immunohistochemical staining of normal fetal and adult skin revealed strong reactivity in basal keratinocytes of epidermis and outer hair root sheath, hair matrix cells and the secretory coil, but not the exocrine portion of eccrine glands; myoepithelial cells around apocrine sweat glands were similarly stained. In epidermal hyperplasia, variable numbers of suprabasal keratinocytes were stained, whereas in interface dermatitis, interrupted staining was found in the basal layer. Weak or scattered staining was observed in squamous tumours, whereas basal cell carcinomas did not show consistent staining. The distribution of MT in normal skin was in line with the germinative role of basal keratinocytes and hair matrix cells, whereas its distribution in hyperplastic epidermis was in line with experimental animal data, and reflected the increase in the germinative pool in these conditions. It is concluded that monoclonal antibody L2E3 may serve as a valuable immunohistochemical marker in diagnostic cutaneous pathology since it labels basal keratinocytes selectively, and since it discriminates between eccrine and apocrine sweat glands.