EFFECTS OF CHOLINERGIC DRUGS ON RECEPTIVE-FIELD PROPERTIES OF RABBIT RETINAL GANGLION-CELLS
EFFECTS OF CHOLINERGIC DRUGS ON RECEPTIVE-FIELD PROPERTIES OF RABBIT RETINAL GANGLION-CELLS
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DOI：
10.1113/jphysiol.1982.sp014104
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发表时间：
1982-01-01
影响因子：
5.5
通讯作者：
DAW, NW
中科院分区：
文献类型：
--
作者：
ARIEL, M;DAW, NW
本实验在兔眼原位细胞外记录视网膜神经节细胞感受野特性，研究胆碱能药物对视网膜神经节细胞感受野特性的影响。毒扁豆碱，一种乙酰胆碱酯酶抑制剂，和尼古丁增加了几乎所有类型的视网膜神经节细胞的自发活动。毒扁豆碱的有效性大致与神经元固有的自发活动水平相关。活跃的细胞，具有高的自发放电率，表现出大幅增加，在其维持放电，而呆滞的细胞，很少或没有自发尖峰，表现出小，有时是短暂的增加，在毒扁豆碱的自发活动。神经节细胞对最佳大小和位置的斑点的敏感性在毒扁豆碱的输注过程中没有实质性变化。对光的反应性（高于自发水平的每个刺激的尖峰数量）增加。这种效应发生在缓慢和更复杂的细胞中，很少发生在活跃的细胞中。毒扁豆碱对反应迟钝和更复杂的细胞的另一个作用是使这些细胞开-关。对不适当的对比度变化的额外反应具有较长的潜伏期，并且缺乏初始瞬时爆发。复合感受野特性，如方向敏感性，径向光栅抑制，速度调谐和大小特异性也进行了检查。在毒扁豆碱输注过程中，这些抑制特性仍然存在，并且在大多数情况下，每种细胞类型的触发特征仍然存在。这些结果与ACh [乙酰胆碱]从视网膜释放的药理学结果一致。似乎有两种类型的ACh释放，对不同类别的细胞具有最强大的影响。一种释放（瞬时）发生在光的开始和结束，主要作用于迟缓和更复杂的神经节细胞;另一种释放（强直）不是光调制的，主要作用于活跃的细胞。ACh电池的接线图的建议。
Retinal ganglion cells were recorded extracellularly from the rabbit''s eye in situ to study the effects of cholinergic drugs on receptive field properties. Physostigmine, an acetylcholinesterase inhibitor, and nicotine increased the spontaneous activity of nearly all retinal ganglion cell types. The effectiveness of physostigmine was roughly correlated with the neuron''s inherent level of spontaneous activity. Brisk cells, having high rates of spontaneous firing, showed large increases in their maintained discharge, whereas sluggish cells, with few or no spontaneous spikes, showed small and sometimes transient increases in spontaneous activity during physostigmine. The sensitivity of ganglion cells to spots of optimal size and position did not change substantially during the infusion of physostigmine. The responsiveness to light (number of spikes per stimulus above the spontaneous level) increased. This effect occurred with sluggish and more complex cells, rarely with brisk cells. Another effect of physostigmine on sluggish and more complex cells was to make these cells on-off. The additional response to the inappropriate change in contrast had a long latency and lacked an initial transient burst. Complex receptive field properties such as orientation sensitivity, radial grating inhibition, speed tuning and size specificity were also examined. These inhibitory properties were still present during infusion of physostigmine and, in most cases, the trigger feature of each cell type remained. These results are consistent with pharmacological results on ACh [acetylcholine] release from the retina. There appear to be 2 types of release of ACh, having their most powerful influences on separate classes of cells. One release (transient) occurs at light onset and offset and acts primarily on sluggish and more complex ganglion cells; the other release (tonic) is not light-modulated and acts primarily on brisk cells. A wiring diagram for the ACh cells is suggested.