Proenkephalin messenger RNA is expressed both in the rat anterior and posterior pituitary.
Proenkephalin messenger RNA is expressed both in the rat anterior and posterior pituitary.
复制标题
脑啡肽原信使 RNA 在大鼠垂体前叶和后叶中均表达。
DOI：
10.1159/000125372
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发表时间：
1990
影响因子：
4.1
通讯作者：
Watson,SJ
中科院分区：
文献类型：
--
作者：
Schäfer,MK;Day,R;Ortega,MR;Akil,H;Watson,SJ
众所周知，脑垂体中存在脑啡肽原 (PENK) 衍生的阿片肽。然而，所有三个垂体叶中其生物合成来源的细胞来源尚不清楚。在本研究中，我们通过使用原位杂交组织化学定位大鼠垂体中编码 PENK 的 mRNA，确定了潜在的合成位点。在整个前叶检测到大量含有 PENK mRNA 的细胞。尽管之前的报告表明，在中间叶中没有检测到 mRNA 信号。令人惊讶的是，在后叶中发现了高水平的 PENK mRNA。神经叶中的细胞分布表明垂体细胞（一类特殊的神经胶质细胞）可能表达 PENK mRNA。
The presence of proenkephalin (PENK)-derived opioid peptides in the pituitary gland is well known. However, the cellular sources of their biosynthetic origin in all three pituitary lobes are less clear. In this study we identified the potential sites of synthesis by localizing the mRNA coding for PENK in the rat pituitary gland using in situ hybridization histochemistry. Numerous cells containing PENK mRNA were detected throughout the anterior lobe. Although suggested by previous reports, no mRNA signal could be detected in the intermediate lobe. Surprisingly, high levels of PENK mRNA were found in the posterior lobe. The cellular distribution in the neural lobe implies that pituicytes, a special class of glial cells, may express PENK mRNA.