GENE-DOSE TITRATION ANALYSIS IN THE SEARCH OF TRANS-REGULATORY GENES IN DROSOPHILA
GENE-DOSE TITRATION ANALYSIS IN THE SEARCH OF TRANS-REGULATORY GENES IN DROSOPHILA
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DOI：
10.1002/j.1460-2075.1982.tb01165.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
GARCIABELLIDO, A
中科院分区：
文献类型：
--
作者：
BOTAS, J;DELPRADO, JM;GARCIABELLIDO, A
在果蝇的双胸复合体（BX-C）和棘刺-鳞片复合体（AS-C）两个遗传系统中寻找了反调控基因。先前的遗传证据表明，BX-C和AS-C的激活都取决于反调节基因（polycomb, Pc，前者和hairy， h，后者）在阴性控制中起作用。这些调控基因在杂合条件下的突变体在具有额外剂量的相应基因复合物的果蝇中具有显性的抑制表型。研究人员还寻找了具有类似基因剂量关系的新基因座。BX-C实验中只分离到新的Pc等位基因（6个）。在AS-C实验中发现了4个等位基因和13个新位点（examacrochetae, emc）的等位基因。而h位点显示出特定的相互作用，新的位点，emc，是特定于AS-C的鳞片部分。在这两种系统中，这些可能是基因组中唯一具有剂量依赖性的基因座。
Trans-regulatory genes were sought in 2 genetic systems in Drosophila, the bithorax complex (BX-C) and the achete-scute complex (AS-C). Previous genetic evidence suggests that the activation of both BX-C and AS-C depends on trans-regulatory genes (polycomb, Pc, in the former and hairy, h, in the latter) acting in a negative type of control. Mutants of these regulatory genes in the heterozygous condition have dominant derepression phenotypes in flies with extra doses of the corresponding gene complexes. New loci, with similar gene-dose relationships, were also sought. Only new alleles (6) of Pc were isolated in the BX-C experiment. In the AS-C experiment 4 h alleles, and 13 alleles of a new locus (extramacrochetae, emc) were discovered. Whereas the h locus shows specific interactions upon achete, the new locus, emc, is specific for the scute part of the AS-C. These are probably the only loci in the genome with those dose-dependent properties in the 2 systems.