SEQUENCE-SPECIFIC INHIBITION OF RNA ELONGATION BY ACTINOMYCIN-D
SEQUENCE-SPECIFIC INHIBITION OF RNA ELONGATION BY ACTINOMYCIN-D
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DOI：
10.1016/0014-5793(83)80950-8
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发表时间：
1983-01-01
期刊：
影响因子：
3.5
通讯作者：
BEABEALASHVILLI, RS
中科院分区：
文献类型：
--
作者：
AIVASASHVILLI, VA;BEABEALASHVILLI, RS
我们已经开发了一种方法来定位特定的位点，其中RNA延伸由于DNA结合的配体而被阻止。用该方法测定了放线菌素D的表观结合位点。在低浓度放线菌素D条件下，我们在T7和D111 T7 DNA全长380个核苷酸的核苷酸序列上发现了14个沿着强RNA阻碍位点。所有位点的核苷酸序列由通式XGCY描述，其中X为G，Y为C。
We have developed a method to localize specific sites where RNA elongation is arrested due to DNA‐bound ligands. The method was used to determine apparent binding sites for actinomycin D. We have found 14 strong RNA hindrance sites along nucleotide sequence of T7 and D111 T7 DNA of 380 nucleotides full length under low actinomycin D concentration conditions. Nucleotide sequence of all the sites is described by general formula XGCY where X ‡ G and Y ‡ C.