AN EOSINOPHIL LEUKOCYTE CHEMOTACTIC FACTOR OF ANAPHYLAXIS
AN EOSINOPHIL LEUKOCYTE CHEMOTACTIC FACTOR OF ANAPHYLAXIS
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过敏反应的嗜酸性粒细胞趋化因子
DOI：
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发表时间：
1971
影响因子：
15.3
通讯作者：
K. F. Austen
中科院分区：
文献类型：
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作者：
A. B. Kay;Daniel J. Stechschulte;K. F. Austen
主动或被动致敏的豚鼠肺与抗原发生反应以释放对嗜酸性粒细胞具有特异性趋化作用的因子（ECF-A）的能力已被证明。 ECF-A的释放还伴随着组胺和SRS-A的产生，并且所有这些介质的出现在被动致敏的时间进程、抗原剂量的影响、释放的时间进程、二价阳离子依赖性以及琥珀酸盐或马来酸盐的存在的增强方面表现出相似的反应。通过施用纯化的眼镜蛇毒因子进行去补体对抗原诱导的主动或被动致敏肺碎片中 ECF-A 的释放没有影响。当用豚鼠 7S IgG 级分被动致敏豚鼠肺碎片时，只有含有 IgG1 的级分才能制备用于抗原诱导 ECF-A 释放的组织。组胺、SRS-A、缓激肽、血清素和前列腺素 PGE1、PGE2 和 PGF2α 本身不具有嗜酸性粒细胞作用；在没有抗原的情况下将这些试剂与致敏肺一起孵育后，也没有检测到 ECF-A。嗜酸性粒细胞活性和 SRS-A 在 80% 乙醇中提取并蒸发至干后均保持不变。然而，SRS-A 在碱性溶液中经受煮沸 20 分钟，而 ECF-A 活性在此过程中被消除。 SRS-A和ECF-A也可以通过凝胶过滤来分离。 ECF-A 的活性在通过 Sephadex G-25 柱后完全恢复，估计分子量在 500 至 1000 之间。根据大小和独立于补体系统的形成机制，ECF-A 与之前描述的补体依赖性嗜酸性粒细胞因子 (ECF-C) 不同。因此，ECF-A代表了迄今为止未描述的选择性吸引嗜酸性粒细胞的试剂。
The capacity of actively or passively sensitized guinea pig lung to react with antigen to release a factor specifically chemotactic for eosinophil leukocytes (ECF-A) has been demonstrated. The release of ECF-A was also accompanied by the elaboration of both histamine and SRS-A and the appearance of all these mediators exhibited a similar response in terms of the time course of passve sensitization, the effect of antigen dose, the time course of release, divalent cation dependence and enhancement by the presence of succinate or maleate. Decomplementation by the administration of purified cobra venom factor had no effect on the antigen-induced release of ECF-A from actively or passively sensitized lung fragments. When fragments of guinea pig lung were passively sensitized with fractions of guinea pig 7S IgG, only the IgG1-containing fractions prepared tissue for the antigen-induced release of ECF-A. Histamine, SRS-A, bradykinin, serotonin, and the prostaglandins PGE1, PGE2, and PGF2α were not eosinophilotactic per se; neither was ECF-A detected following the incubation of these agents with sensitized lung in the absence of antigen. Both eosinophilotactic activity and SRS-A survived extraction in 80% ethanol and evaporation to dryness. SRS-A, however, withstood boiling in alkaline solution for 20 min, whereas ECF-A activity was abolished by this procedure. SRS-A and ECF-A could also be separated by gel filtration. ECF-A activity was completely recovered following its passage through a column of Sephadex G-25 and had an estimated molecular weight of between 500 and 1000. On the basis of size and a formation mechanism independent of the complement system, ECF-A is distinguishable from a previously described complement-dependent eosinophilotactic factor (ECF-C). Thus, ECF-A represents a hitherto undescribed agent which selectively attracts eosinophil leukocytes.