Analysis of benzene ethylamine derivatives in urine using the programmable dynamic liquid-phase microextraction (LPME) device.
Analysis of benzene ethylamine derivatives in urine using the programmable dynamic liquid-phase microextraction (LPME) device.
复制标题
使用可编程动态液相微萃取 (LPME) 装置分析尿液中的苯乙胺衍生物。
DOI：
10.1039/b312227c
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Myungsoo Kim
中科院分区：
文献类型：
--
作者：
S. Myung;Sung;Myungsoo Kim
制造了用于动态LPME方法的自动化LPME装置，并使用加标尿样测试其提取效率。研制的国产LPME装置是一种可编程的自动注射器分配器，以克服精度下降和手动重复操作柱塞的困难。为了建立苯乙胺的最佳参数，取样体积，溶剂体积，pH值，盐效应，溶剂的选择，柱塞速度和取样次数的影响进行了研究。在3 μ g mL（-1）浓度下，美芬丁胺、麻黄碱、甲氧那明、司来吉兰和安非他酮的提取重复性好，RSD值分别为2.4、1.9、1.3、1.6和1.5%。对于所研究的药物，检测限低于0.05 μ g mL（-1）。该装置用于LPME分析，验证结果良好，方便性提高。
An automated LPME device for a dynamic LPME method was manufactured and its extraction efficiency was tested using spiked urine samples. The developed home-made LPME device was a programmable automated syringe dispenser to overcome deteriorating precision and difficulties in manually manipulating the plunger repeatedly. To establish the optimum parameters for benzene ethylamines, the effects of sampling volume, solvent volume, pH, salt-effect, choice of solvents, plunger speed, and number of samplings were investigated. Good repeatabilities for the extraction of mephentermine, ephedrine, methoxyphenamine, selegiline, and bupropion were obtained and the RSD values were 2.4, 1.9, 1.3, 1.6 and 1.5% at a concentration of 3 microg mL(-1) in spiked urine samples, respectively. The limit of detection was below 0.05 microg mL(-1) for the investigated drugs. This developed device for LPME analysis gave good validation results and improved convenience.