The MC1R gene in the guppy (Poecilia reticulata): Genotypic and phenotypic polymorphisms.
The MC1R gene in the guppy (Poecilia reticulata): Genotypic and phenotypic polymorphisms.
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DOI：
10.1186/1756-0500-4-31
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发表时间：
2011-02-04
影响因子：
1.8
通讯作者：
Kawata M
中科院分区：
文献类型：
--
作者：
Tezuka A;Yamamoto H;Yokoyama J;van Oosterhout C;Kawata M
孔雀鱼（Poecilia reticulata）是研究性选择的重要模式生物;雄性孔雀鱼具有复杂而明显的色素沉着，雌性孔雀鱼对具有特定颜色斑点的雄性表现出偏好。了解孔雀鱼色素变异的遗传基础，对于探索野生种群维持色素多态性的因素具有重要意义。我们专注于黑色素沉着的孔雀鱼，并检查黑皮质素1受体（MC 1 R）基因的遗传变异，因为已知该基因的变异有助于在几种动物中的黑色素沉着的多态性。对孔雀鱼MC 1 R基因的编码序列进行了测定，发现野生群体中存在两个不同的MC 1 R等位基因（963和969 bp）。观赏品系孔雀鱼与963 bp的MC 1 R往往表现出较少的黑色色素沉着比那些与969 bp的MC 1 R，虽然MC 1 R基因型和黑色色素沉着之间的关联消失在F2后代。孔雀鱼MC 1 R基因在我们研究的五个野生特立尼达种群中表现出变异，这些种群的等位基因频率也不同。我们确定了一个显着的关联黑色色素沉着和MC 1 R基因型的鱼从水族馆商店。然而，来自F2家族的结果表明，还有其他基因修饰MC 1 R基因的作用。
The guppy (Poecilia reticulata) is an important model organism for studying sexual selection; male guppies have complex and conspicuous pigmentation, and female guppies exhibit preferences for males with specific color spots. Understanding the genetic basis underlying pigmentation variation in the guppy is important for exploring the factors causing the maintenance of color polymorphism in wild populations. We focused on the melanic black pigmentation of guppies, and examined genetic variations in the melanocortin 1 receptor (MC1R) gene because variation in this gene is known to contribute to polymorphism of melanin pigmentation in several animal species. The complete coding sequence of the guppy MC1R gene was determined, and two different MC1R alleles (963 and 969 bp) were found in wild populations. Ornamental strain guppies with a 963-bp MC1R tended to show less black pigmentation than those with a 969-bp MC1R, although the association between MC1R genotype and black pigmentation disappeared in the F2 offspring. The guppy MC1R gene showed variation in the five wild Trinidadian populations we examined, and these populations also differed in terms of allele frequencies. We identified a significant association between black pigmentation and MC1R genotype in fish obtained from aquarium shops. However, the results from F2 families suggest that there are other genes that modify the effects of the MC1R gene.