Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings
Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings
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DOI：
10.1007/bf00776646
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发表时间：
2001-01-01
影响因子：
2.9
通讯作者：
Sakai, F
中科院分区：
文献类型：
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作者：
Kodan, A;Kuroda, H;Sakai, F
分析了赤松芪合酶（STS）的cDNA序列。从构建的根cDNA文库中分离得到3个新的STS cDNA克隆（pdsts1、pdsts2、pdsts3），它们均携带完整的STS编码序列。其编码区的同源性约为95%，并具有保守的STS基序。并对它们的系统发育关系进行了讨论。用聚合酶链反应扩增STS cDNA片段的3'端，并进行亚克隆。片段的3'非翻译区（3'UTR）突出了结构多样性。12个STS cDNA克隆根据3'UTR序列分为7个不同的亚类。我们讨论了转录本的稳定性及其基因组织。STS成员的同时表达是松树随时间发展的适应机制之一。
We analyzed sequences of the stilbene synthase (STS) cDNA in Pinus densiflora. Three novel STS cDNA clones (pdsts1, pdsts2, pdsts3) that carry full coding sequences were isolated from a cDNA library constructed from the roots. The homologies in their coding regions were about 95%, and they have a conserved STS motif. Their phylogenetic relation was also discussed. The 3' end of the STS cDNA fragments was amplified by the polymerase chain reaction and subcloned. The 3' untranslated region (3'UTR) of the fragments highlighted the structure diversity. Twelve STS cDNA clones were categorized into seven distinct subclasses according to 3'UTR sequences. We discussed the stability of the transcripts and their gene organization. The simultaneous expression of the STS members is one of the mechanisms for adaptation that pine trees have developed over time.