Single-cell recording from the brain of freely moving monkeys.
Single-cell recording from the brain of freely moving monkeys.
复制标题
来自自由活动猴子大脑的单细胞记录。
DOI：
10.1016/s0165-0270(01)00348-x
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发表时间：
2001
影响因子：
3
通讯作者：
Kral,JG
中科院分区：
文献类型：
--
作者：
Ludvig,N;Botero,JM;Tang,HM;Gohil,B;Kral,JG
非人类灵长类动物大脑的单细胞记录传统上是在坐在灵长类动物椅子上的猴子身上进行的。然而，这种安排使得长期记录变得困难，导致可能混淆数据的压力，并阻止自然行为的表现。扩展我们以前在非人灵长类动物中的神经生理学研究（Ludvig等，Brain Res. Protocols 2000;5：75-85），我们开发了一种记录自由移动的松鼠猴（Saimiri sciureus）中单个海马神经元电活动的方法。记录持续了6个小时，在此期间，猴子在一个大型测试室的墙壁和地板上自由移动，并收集食物颗粒。在整个过程中，容易保持稳定的动作电位波形。在这项研究中，以下因素被证明是至关重要的：（a）选择松鼠猴进行实验，（B）使用可驱动的一束微丝进行记录，（c）使用特殊的记录电缆，（d）将微丝植入大脑而不引起神经功能障碍，（e）在特殊的测试室中进行记录。所描述的方法允许长期的细胞外记录从非人类灵长类动物的大脑，没有椅子的压力，在广泛的自然行为。使用这个模型，可以获得新的见解到灵长类动物大脑的神经元的独特的发射剧目。
Single-cell recording from the brain of non-human primates has traditionally been performed in monkeys seated in a primate chair. However, this arrangement makes long-term recordings difficult, causes stress that may confound the data, and prevents the manifestation of natural behaviors. Extending our previous neurophysiological studies in non-human primates (Ludvig et al. Brain Res. Protocols 2000;5:75–85), we have developed a method for recording the electrical activity of single hippocampal neurons in freely moving squirrel monkeys (Saimiri sciureus). The recording sessions lasted for up to 6 h, during which the monkeys moved freely around on the walls and the floor of a large test chamber and collected food pellets. Stable action potential waveforms were readily kept throughout the sessions. The following factors proved to be critical in this study: (a) selecting squirrel monkeys for the experiments, (b) using a driveable bundle of microwires for the recordings, (c) using a special recording cable, (d) implanting the microwires into the brain without causing neurological deficits, and (e) running the recording sessions in a special test chamber. The described method allows long-term extracellular recordings from the brain of non-human primates, without the stress of chairing, during a wide range of natural behaviors. Using this model, new insights can be obtained into the unique firing repertoire of the neurons of the primate brain.