LACTASE PRODUCTION IN CONTINUOUS CULTURE BY TRICHODERMA REESEI RUT-C30
LACTASE PRODUCTION IN CONTINUOUS CULTURE BY TRICHODERMA REESEI RUT-C30
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DOI：
10.1007/bf00135688
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
WILKE, CR
中科院分区：
文献类型：
--
作者：
CASTILLO, FJ;BLANCH, HW;WILKE, CR
T.当在乳糖培养基上生长时，里氏Rut-C30产生胞外乳糖酶。在0.02和0.027 h-1之间的稀释率（D）下观察到连续培养物中的最大酶水平。在D = 0.027 h-1时，酶的生产率达到27.3 U/l h。乳糖酶的合成似乎是可诱导的，并受到分解代谢物的抑制。最适生长温度为28 ℃，最适pH为2.5。C和pH 5。在63 ℃观察到最大酶活性。C和pH 4.6。表观Km，基于硝基苯基-吡喃半乳糖苷测定，估计为0.4 mM。该酶适用于酸乳清中乳糖的水解。
T. reesei Rut-C30 produces extracellular lactase when grown on lactose medium. Maximum enzyme levels in continuous culture were observed at dilution rates (D) between 0.02 and 0.027 h-1. The enzyme productivity reached 27.3 U/l h at D = 0.027 h-1. Lactase synthesis appears to be inducible and subject to catabolite repression. Optimal growth temperature and pH for enyzme production were 28.degree. C and pH 5. Maximum enzyme activity was observed at 63.degree. C and pH 4.6. The apparent Km, based on the nitrophenyl-galactopyranoside assay, was estimated as 0.4 mM. The enyzme is suitable for lactose hydrolysis in acid whey.