VERTICAL TRANSMISSION OF WEST NILE VIRUS BY CULEX AND AEDES SPECIES MOSQUITOS
VERTICAL TRANSMISSION OF WEST NILE VIRUS BY CULEX AND AEDES SPECIES MOSQUITOS
复制标题
DOI：
10.4269/ajtmh.1993.48.757
复制
发表时间：
1993-06-01
影响因子：
3.3
通讯作者：
WATTS, DM
中科院分区：
文献类型：
--
作者：
BAQAR, S;HAYES, CG;WATTS, DM
进行实验以确定西尼罗河 (WN) 病毒是否通过定植的白纹伊蚊菌株垂直传播。埃及伊蚊和三带喙库蚊。通过胸腔内接种WN病毒感染雌蚊，并通过荧光抗体技术和新生小鼠试验对F1后代进行病毒检测。这三种蚊子均将 WN 病毒传播给来自 26 摄氏度下饲养的未成熟蚊子的 F1 成虫。伊蚊的最小子代感染率 (MFIR) 范围为 1:124 至 1:138。白纹伊蚊，Ae 从 1:62 到 1:172。埃及伊蚊，Cx 为 1:325 至 1:859。三带喙鸟。 Cx 的 MFIR。 20℃饲养的三带喙鱼幼虫和蛹的比例为1:213，26℃饲养的幼虫和蛹的比例为1:208和1:554。这些数据是首次报告的 WN 病毒通过蚊子垂直传播的证据，因此需要进一步研究以确定自然界中感染 WN 病毒的蚊子之间是否发生垂直传播。
Experiments were conducted to determine whether West Nile (WN) virus was transmitted vertically by colonized strains of Aedes albopictus, Ae. aegypti, and Culex tritaeniorhynchus. Female mosquitoes were infected by intrathoracic inoculation with WN virus, and the F1 progeny were tested for virus by the fluorescence antibody technique and the newborn mouse assay. Each of the three mosquito species transmitted WN virus to F1 adults derived from immature forms reared at 26-degrees-C. The minimal filial infection rate (MFIR) ranged from 1:124 to 1:138 for Ae. albopictus, from 1:62 to 1:172 for Ae. aegypti, and from 1:325 to 1:859 for Cx. tritaeniorhynchus. The MFIR for Cx. tritaeniorhynchus reared at 20-degrees-C was 1:213 for larvae and 1:390 for pupae, and 1:208 for larvae and 1:554 for pupae reared at 26-degrees-C. These data are the first reported evidence of vertical transmission of WN virus by mosquitoes, and therefore warrant further studies to determine whether vertical transmission occurs among WN viral-infected mosquitoes in nature.