The effect of doxorubicin on rats that received toxic and carcinogenic benzo(a)pyrene
The effect of doxorubicin on rats that received toxic and carcinogenic benzo(a)pyrene
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DOI：
10.2478/v10042-008-0048-x
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发表时间：
2008-01-01
影响因子：
1.5
通讯作者：
Aral, Erinc
中科院分区：
文献类型：
--
作者：
Cosan, Didem;Basaran, Ayse;Aral, Erinc
苯并(a)芘(B(a) P)是一种多环芳香烃，具有致癌和毒性作用。阿霉素是一种广泛用于化疗的 DNA 相互作用药物。在本研究中，我们研究了阿霉素对接受苯并（a）芘的大鼠的影响。 Sprague-Dawley雄性大鼠，3-4月龄，分为5组（每组n=9）。第1组（对照组）腹膜内（i.p.）和胃内（i.p.）接受生理盐水，第2组（对照组）同样接受玉米油腹膜内注射。例如，第 3 组接受玉米油溶性苯并（a）芘（每 10 天 10 mg/kg b.wt，持续 40 天），第 4 组接受阿霉素（连续 3 天腹腔注射 4 mg），第 5 组接受阿霉素 3 天（如第 4 组），然后如第 3 组接受苯并（a）芘。24 小时后收集尿液样本，收集心脏血液、肝脏和肾脏组织获得样品。对尿液和血液的生化数据进行评估；对肝脏和肾脏组织样本进行组织学研究。第三组的尿酸、尿肌酐、肌酸清除率、尿素氮、血清肌酐值、血清谷草酰乙酸转氨酶（SGOT、AST）、血清谷氨酸丙酮转氨酶（SGPT、ALT）、碱性磷酸酶（ALP、AP）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和丙二醛（MDA）水平与对照组相比显着差异组。第 5 组的大多数参数在统计上与对照值相似。肝脏和肾脏组织样本的组织学外观支持第五组的改善。我们的研究结果表明，因苯并（a）芘而受损的肝肾功能可以通过阿霉素部分恢复。
Benzo(a) pyrene (B(a) P) is a polycyclic aromatic hydrocarbon with carcinogenic and toxic effects. Doxorubicin is a DNA-interacting drug widely used in chemotherapy. In the present study we investigated the effects of doxorubicin on rats that received benzo(a) pyrene. Sprague-Dawley male rats, 3-4 months old, were divided into 5 groups (n=9 per group). Group 1 (controls) received normal saline intraperitoneally (i.p.) and intragastrically (i.g.), Group 2 (controls) similarly received corn oil i.p. and i.g., Group 3 received corn oil soluble benzo(a) pyrene (10 mg/kg b.wt every 10 days for 40 days), Group 4 received doxorubicin (4mg i.p. on 3 consecutive days), Group 5 received doxorubicin for 3 days (as in group 4) followed by benzo(a) pyrene as in group 3. After twenty-four hours urine samples were collected, heart blood, liver and kidney tissue samples were obtained. Biochemical data were evaluated on urine and blood; liver and kidney tissue samples were investigated histologically. Uric acid, urine creatinine, creatine clearance, urea nitrogen, serum creatinine values, serum glutamic oxaloacetic transaminase (SGOT, AST), serum glutamic pyruvic transaminase (SGPT, ALT), alkaline phosphatase (ALP, AP), superoxide dismutase (SOD), catalase (CAT) activities and malondialdehyde (MDA) levels were significantly different in the 3rd group compared with control groups. Most of the parameters group 5 were statistically similar to control values. Histological appearance of the liver and the kidney tissue samples supported the improvement in the 5th group. The result of our study indicated that liver and kidney functions impaired with benzo(a) pyrene may be partially restored by doxorubicin.