Signal transduction by human herpesvirus 8 viral interleukin-6 (vIL-6) is modulated by the nonsignaling gp80 subunit of the IL-6 receptor complex and is distinct from signaling induced by human IL-6
Signal transduction by human herpesvirus 8 viral interleukin-6 (vIL-6) is modulated by the nonsignaling gp80 subunit of the IL-6 receptor complex and is distinct from signaling induced by human IL-6
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DOI：
10.1128/jvi.00767-06
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Nicholas, John
中科院分区：
文献类型：
--
作者：
Hu, Fang;Nicholas, John
人疱疹病毒8（HHV-8）病毒白细胞介素-6（vIL-6）通过gp 130信号转导介导信号传导，但与人IL-6（hIL-6）不同，其不需要IL-6受体复合物的非信号传导gp 80 α亚基。通过利用gp 80难治性vIL-6变体vIL-6（R189 L），我们发现信号转导，如通过STAT 1测量的。在gp 80（+）/gp 130（+）HEK 293 T细胞中，gp 80调节STAT 3活化和gp 130酪氨酸磷酸化。此外，vIL-6和hIL-6的信号传导和BAF-130细胞生长促进活性可以区分，并且外源性添加可溶性gp 80增强vIL-6支持的细胞生长。我们的研究结果表明，gp 80可以调节vIL-6的活性和vIL-6和hIL-6信号不是直接等效的。
Human herpesvirus 8 (HHV-8) viral interleukin-6 (vlL-6) mediates signaling through the gp130 signal transducer but unlike human IL-6 (hIL-6) does not require the nonsignaling gp80 alpha subunit of the IL-6 receptor complex. By utilizing a gp80-refractory vIL-6 variant, vIL-6(R189L), we found that signal transduction, as measured by STAT1. and STAT3 activation and gp130 tyrosine phosphorylation in gp80(+)/gp130(+) HEK293T cells, was modulated by gp80. Furthermore, the signaling and BAF-130 cell growth-promoting activities of vIL-6 and hIL-6 could be distinguished, and exogenous addition of soluble gp80 enhanced cell growth supported by vIL-6. Our findings demonstrate that gp80 can modulate vIL-6 activity and that vIL-6 and hIL-6 signaling are not directly equivalent.