Intrinsic innervation in the intestine of the lizard Podarcis hispanica
Intrinsic innervation in the intestine of the lizard Podarcis hispanica
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DOI：
10.14670/hh-15.1093
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发表时间：
2000-10-01
影响因子：
2
通讯作者：
Blasco, J
中科院分区：
文献类型：
--
作者：
Martinez-Ciriano, C;Junquera, C;Blasco, J
本研究的目的是用不同的组织化学方法描述足蜥肠道神经结构成分的形态和分布;即乙酰胆碱酯酶（AChE）、甲醛诱导的儿茶酚胺荧光（FIF）、烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）和血管活性肠肽（VIP）的免疫组织化学，以及P物质（SP）和电子显微镜。乙酰胆碱酯酶法显示肌间神经丛和粘膜下神经丛中的纤维，大肠中的纤维密度较高。最高数量的相关神经元位于肌间神经丛神经节。去甲肾上腺素能神经分布通过肌间神经丛和粘膜下神经丛，血管周围，在大肠中的纤维密度最高。VIP免疫组化显示，广泛分布的阳性纤维在整个肠道，虽然最高密度再次检测到大肠。VIP阳性细胞主要分布于神经丛的结间节段。SP标记，虽然微妙，是目前所有沿着肠。它显示了精致的静脉曲张网和很少的纤维支配血管。大肠内可见少量SP阳性细胞。间接法检测一氧化氮（NO）生成系统显示大肠肌间神经丛神经节内有神经细胞。电子显微镜下可见神经节细胞染色质凝聚，电子密度较高，轴突有不同的囊泡。我们还确定了某些细胞作为卡哈尔间质细胞，由于其超微结构特征。它们大多位于肌间神经丛区域。
The aim of this study was the description of the morphology and distribution of nerve structure elements in the intestine of the lizard Podarcis hispanica using different histochemical methods; namely acetylcholinesterase (AChE), formol-induced fluorescence for catecholamines (FIF), nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), and immunohistochemistry for vasoactive intestinal peptide (VIP), as well as substance P (SP) and electron microscopy. The AChE method showed fibres in the myenteric and submucosal plexus, with a higher fibre density in the large intestine. The highest number of related neurons was located in the myenteric plexus ganglia. Noradrenergic innervation was distributed through the myenteric and submucosal plexus, and also around blood vessels, with the highest fibre density in the large intestine. VIP immunohistochemistry showed a wide distribution of positive fibres throughout the intestine, although the highest density was again detected in the large intestine. Small positive cells for VIP were located at internodal segments in the plexus. SP labeling, although subtle, was present all along the intestine. It showed delicate varicose nets and few fibres innervating blood vessels. Small positive cells for SP were located in the large intestine. The indirect method to detect nitric oxide (NO)-producing system showed neural cells in the myenteric plexus ganglia of the large intestine. Electron microscopy showed ganglion neurons with scattered chromatin condensations, glial cells with higher electron density, and axons with varicosities occupied by different vesicles. We also identified certain cells as interstitial cells of Cajal due to their ultrastructural features. They were mostly located in the region of the myenteric plexus.